Role of Plc1p in regulation of Mcm1p-dependent genes.
Role of Plc1p in regulation of Mcm1p-dependent genes.
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Plc1p 在 Mcm1p 依赖性基因调节中的作用。
DOI：
10.1111/j.1574-6968.2009.01602.x
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发表时间：
2009
影响因子：
2.1
通讯作者：
Vancura,Ales
中科院分区：
文献类型：
--
作者：
Guzinska,Katarzyna;Varghese,Roger;Vancura,Ales
在芽殖酵母中，磷酸肌醇特异性磷脂酶 C（由 PLC1 基因编码的 Plc1p）和几种肌醇多磷酸激酶代表了肌醇多磷酸 (InsPs) 合成的核途径，其参与 DNA 和 RNA 代谢的多个方面，包括转录调控。 Plc1p 产生的肌醇三磷酸 (InsP3) 被 Ipk2p/Arg82p 磷酸化，产生 InsP4/InsP5。 Ipk2p/Arg82p 也是 ArgR-Mcm1p 复合物的一个组成部分，调节参与精氨酸代谢的基因的转录。 Ipk2p/Arg82p 在该复合物中的作用是稳定必需的 MADS 盒蛋白 Mcm1p。因此，ipk2Δ细胞表现出Mcm1p水平降低和Mcm1p依赖性基因的表达减弱。由于plc1Δ细胞表现出几组基因的异常表达，包括参与应激反应的基因，本研究的目的是确定Plc1p是否也影响Mcm1p依赖性基因的表达。在这里，我们报告不仅 ipk2Δ，而且 plc1Δ 细胞也显示出 Mcm1p 依赖性基因的表达下降。然而，Plc1p 不参与 Mcm1p 的稳定，并通过不同的机制影响 Mcm1p 依赖性基因的转录，可能涉及染色质重塑复合物的调节。
In budding yeasts, phosphoinositide-specific phospholipase C (Plc1p encoded byPLC1gene) and several inositol polyphosphate kinases represent a nuclear pathway for synthesis of inositol polyphosphates (InsPs), which are involved in several aspects of DNA and RNA metabolism, including transcriptional regulation. Plc1p-produced inositol trisphosphate (InsP3) is phosphorylated by Ipk2p/Arg82p to yield InsP4/InsP5. Ipk2p/Arg82p is also a component of ArgR-Mcm1p complex that regulates transcription of genes involved in arginine metabolism. The role of Ipk2p/Arg82p in this complex is to stabilize the essential MADS box protein Mcm1p. Consequently,ipk2Δ cells display reduced levels of Mcm1p and attenuated expression of Mcm1p-dependent genes. Becauseplc1Δ cells display aberrant expression of several groups of genes, including genes involved in stress response, the objective of this study was to determine whether Plc1p also affects expression of Mcm1p-dependent genes. Here we report that not onlyipk2Δ, but alsoplc1Δ cells display decreased expression of Mcm1p-dependent genes. However, Plc1p is not involved in stabilization of Mcm1p and affects transcription of Mcm1p-dependent genes by a different mechanism, probably involving regulation of chromatin remodeling complexes.