Diversity in the circadian periods of single neurons of the rat suprachiasmatic nucleus depends on nuclear structure and intrinsic period
Diversity in the circadian periods of single neurons of the rat suprachiasmatic nucleus depends on nuclear structure and intrinsic period
复制标题
DOI：
10.1016/j.neulet.2004.01.022
复制
发表时间：
2004-04-01
影响因子：
2.5
通讯作者：
Honma, K
中科院分区：
文献类型：
--
作者：
Honma, S;Nakamura, W;Honma, K
用多电极阵列碟法测定了大鼠下丘脑视交叉上核(SCN)单个培养神经元的昼夜节律。虽然分散细胞培养和器型切片培养的平均昼夜周期没有差异，但前者的周期分布在20.0~30.9个周期的较大范围内，而后者的周期集中在一个较窄的范围内。在每个培养皿中也检测到了相同的差异。周期长度与昼夜节律的变化有显著的相关性，培养皿中的平均昼夜节律周期偏离总体平均值越大，其周期的标准差就越大。这种相关性在器官型切片培养中没有观察到。这些发现表明，单个SCN神经元的昼夜节律周期的多样性取决于SCN结构的维持和昼夜节律周期的维持，这表明不仅细胞间的通讯，而且内在的昼夜节律周期在SCN的结构振荡器的同步化中起着重要作用。(C)2004爱思唯尔爱尔兰有限公司。保留所有权利。
The circadian periods of single cultured neurons of the hypothalamic suprachiasmatic nucleus (SCN) in rats were assessed by means of multi-electrode array dish. Although the mean circadian period was not different between the dispersed cell culture and organotypic slice culture, the periods distributed in a wide range from 20.0 to 30.9 It in the former whereas concentrated in a narrow range in the latter. The same difference was also detected within each culture dish. There is a significant correlation between the period length and variation of circadian rhythm, where the more the mean circadian period in a culture dish deviates from the overall mean, the larger the standard deviation of period in a dish becomes. Such a correlation was not observed in the organotypic slice culture. These findings indicate that the diversity of circadian periods in the individual SCN neurons depends on the maintenance of SCN structure and the circadian period, suggesting that not only cell-to-cell communication but also the intrinsic circadian period plays a significant role in synchronizing the constitutional oscillators in the SCN. (C) 2004 Elsevier Ireland Ltd. All rights reserved.