The actin-related protein Act3p of Saccharomyces cerevisiae is located in the nucleus.
The actin-related protein Act3p of Saccharomyces cerevisiae is located in the nucleus.
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酿酒酵母的肌动蛋白相关蛋白Act3p位于细胞核中。
DOI：
10.1091/mbc.6.10.1263
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发表时间：
1995
影响因子：
3.3
通讯作者：
U. Wintersberger
中科院分区：
文献类型：
--
作者：
V. Weber;M. Harata;H. Hauser;U. Wintersberger
肌动蛋白相关蛋白是一组相互之间以及与常规肌动蛋白之间具有约50%序列同一性的蛋白质家族，已在多种真核生物中发现。在芽殖酵母酿酒酵母（Saccharomyces cerevisiae）中，已知一种常规肌动蛋白（ACT 1）和三种肌动蛋白相关蛋白（ACT 2、ACT 3和ACT 5）的基因。我们最近发现的ACT 3是编码489个氨基酸的多肽（Act 3 p）的必需基因，计算分子量为54.8 kDa。除了与常规肌动蛋白同源外，Act 3 p还具有与染色体蛋白HMG-14表现出弱相似性的结构域以及潜在的核定位信号。针对ACT 3基因产物的特定片段制备的抗血清识别酵母提取物中约55 kDa的多肽条带。用该抗血清进行的间接免疫荧光实验表明，Act 3 p位于细胞核中。在所有细胞中观察到核染色，无论细胞周期的阶段。独立地，用亚细胞级分的免疫印迹实验表明，Act 3 p确实高度富集在核级分中。我们认为Act 3 p是酵母染色质的重要组成部分。
Actin-related proteins, a group of protein families that exhibit about 50% sequence identity among each other and to conventional actin, have been found in a variety of eukaryotic organisms. In the budding yeast Saccharomyces cerevisiae, genes for one conventional actin (ACT1) and for three actin-related proteins (ACT2, ACT3, and ACT5) are known. ACT3, which we recently discovered, is an essential gene coding for a polypeptide of 489 amino acids (Act3p), with a calculated molecular mass of 54.8 kDa. Besides its homology to conventional actin, Act3p possesses a domain exhibiting weak similarity to the chromosomal protein HMG-14 as well as a potential nuclear localization signal. An antiserum prepared against a specific segment of the ACT3 gene product recognizes a polypeptide band of approximately 55 kDa in yeast extract. Indirect immunofluorescence experiments with this antiserum revealed that Act3p is located in the nucleus. Nuclear staining was observed in all cells regardless of the stage of the cell cycle. Independently, immunoblotting experiments with subcellular fractions showed that Act3p is indeed highly enriched in the nuclear fraction. We suggest that Act3p is an essential constituent of yeast chromatin.
果蝇中不同肌动蛋白相关蛋白的鉴定。
影响因子：
5.6
作者：
Frankel,S;Heintzelman,MB;Artavanis-Tsakonas,S;Mooseker,MS
通讯作者：
Mooseker,MS