The virulence factor PA protein of highly pathogenic H5N1 avian influenza virus inhibits NF-kappa B transcription in vitro
The virulence factor PA protein of highly pathogenic H5N1 avian influenza virus inhibits NF-kappa B transcription in vitro
复制标题
高致病性H5N1禽流感病毒毒力因子PA蛋白体外抑制NF-κB转录
DOI：
10.1007/s00705-017-3496-9
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发表时间：
2017
影响因子：
2.7
通讯作者：
Liu Xiufan
中科院分区：
文献类型：
--
作者：
Cui Zhu;Hu Jiao;Wang Xiaoquan;Gu Min;Liu Xiaowen;Hu Shunlin;Hu Zenglei;Liu Huimou;Liu Wenbo;Chen Sujuan;Peng Daxin;Jiao Xinan;Liu Xiufan
核因子-κB在炎症和免疫反应中起着至关重要的作用。我们之前的研究已经证明，先天免疫反应会影响H5N1病毒在小鼠中的毒力。在本研究中，我们首次发现高致病性禽流感病毒CK10株的PA蛋白对NF-κB活性的抑制作用最强，并且具有剂量依赖性。然后，我们确定了PA的关键氨基酸，这些氨基酸对这一效应有贡献。此外，PA还可抑制由NF-κB调节的炎症因子，包括IL-6、IL-2、NOS-2和α。但PA对NF-κB活化的抑制作用与p65的核转位无关。
Nuclear factor kappa B (NF-κB) plays a crucial role in inflammation and immune responses. Our previous studies have demonstrated that the innate immune response affect H5N1 virus virulence in mice. In this study, we first showed that the PA protein of the highly pathogenic avian influenza virus strain CK10 had the strongest inhibitory effect on NF-κB activation when compared with other genes, and that it acted in a dose independent-manner. We then determined the critical amino acids of PA that contribute to this effect. Furthermore, PA also inhibited NF-κB-regulated inflammatory factors, including IL-6, IL-2, Nos-2 and TNF-α. However, the inhibitory effect on NF-κB activation mediated by PA was not associated with nuclear translocation of p65.