Molecular cloning and characterization of WNT3A and WNT14 clustered in human chromosome 1q42 region
Molecular cloning and characterization of WNT3A and WNT14 clustered in human chromosome 1q42 region
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DOI：
10.1006/bbrc.2001.5105
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发表时间：
2001-06-29
影响因子：
3.1
通讯作者：
Katoh, M
中科院分区：
文献类型：
--
作者：
Saitoh, T;Hirai, M;Katoh, M
克隆并表征了人WNT 3A和WNT 14 cDNA。WNT 3A和WNT 14分别编码352和365个氨基酸的WNT家族蛋白。3.0-kb WNT 3A mRNA在胎盘中中度表达，4.4-kb WNT 14 mRNA在骨骼肌和心脏中中度表达。虽然在35个人癌细胞系中未检测到WNT 3A mRNA，但WNT 14 mRNA在胃癌细胞系TMK 1、MKN 7、MKN 45和KATO-III中表达。利用荧光原位杂交技术将WNT 3A和WNT 14基因定位于人染色体1 q42区域，两个基因以头对头的方式聚集在一起，间距约为58.0kb。WNT 3和WNT 15分别是WNT 3A和WNT 14的相关基因，聚簇于人类染色体17 q21区域。与WNT 10A-WNT 6基因簇和WNT 10 B-WNT 1基因簇一样，WNT 3A-WNT 14基因簇和WNT 3-WNT 15基因簇可能是由于祖先基因簇的复制而产生的。小鼠乳腺肿瘤病毒（MMTV）的整合位点位于与这些人WNT基因簇相对应的小鼠染色体区域。这些结果有力地表明，未鉴定的核苷酸基序负责重组的易感性可能存在于这些WNT基因簇的基因间区域。(C)北京：科学出版社.
Human WNT3A and WNT14 cDNAs were cloned and characterized. WNT3A and WNT14 encoded WNT family protein of 352 and 365 amino acids, respectively. The 3.0-kb WNT3A mRNA was moderately expressed in placenta, and the 4.4-kb WNT14 mRNA was moderately expressed in skeletal muscle and heart. Although WNT3A mRNA was not detected in 35 human cancer cell lines, WNT14 mRNA was expressed in gastric cancer cell lines TMK1, MKN7, MKN45 and KATO-III. WNT3A and WNT14 genes, clustered in the head to head manner with an interval of about 58.0 kb, were mapped to human chromosome 1q42 region by fluorescence in situ hybridization. WNT3 and WNT15, clustered in human chromosome 17q21 region, are related genes of WNT3A and WNT14, respectively. WNT3A-WNT14 gene cluster and WNT3-WNT15 gene cluster might be generated due to duplication of ancestral gene cluster, just like WNT10A-WNT6 gene cluster and WNT10B-WNT1 gene cluster. Integration sites of mouse mammary tumor virus (MMTV) are located in the mouse chromosomal regions corresponding to these human WNT gene clusters. These results strongly suggest that unidentified nucleotide motif responsible for susceptibility to recombination might exist within the intergenic regions of these WNT gene clusters. (C) 2001 Academic Press.