Artificial imine reductases: developments and future directions.
Artificial imine reductases: developments and future directions.
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DOI：
10.1039/d0cb00113a
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发表时间：
2020-12-01
影响因子：
4.1
通讯作者：
Duhme-Klair AK
中科院分区：
文献类型：
--
作者：
Booth RL;Grogan G;Wilson KS;Duhme-Klair AK
生物催化亚胺还原反应是近年来人工金属酶界研究的热点。人工构建体与天然酶一起被工程化以产生具有高对映选择性的手性胺。本文综述了人工亚胺还原酶的主要类别的设计迄今报道，并总结了方法，以提高其催化性能，使用互补的方法。在体内或在多酶级联中利用这些生物催化剂的实例已经证明了artIRED可以提供的潜力，然而，目前它们在生物催化中的用途仍然有限。本文综述了artIRED目前的范围和用于催化剂改进的策略，并探讨了artIRED在未来的潜力。本文综述了这类人工金属酶的发展，并对该领域的现有和未来研究进行了展望。
Biocatalytic imine reduction has been a topic of intense research by the artificial metalloenzyme community in recent years. Artificial constructs, together with natural enzymes, have been engineered to produce chiral amines with high enantioselectivity. This review examines the design of the main classes of artificial imine reductases reported thus far and summarises approaches to enhancing their catalytic performance using complementary methods. Examples of utilising these biocatalysts in vivo or in multi-enzyme cascades have demonstrated the potential that artIREDs can offer, however, at this time their use in biocatalysis remains limited. This review explores the current scope of artIREDs and the strategies used for catalyst improvement, and examines the potential for artIREDs in the future. This review surveys the development of this class of artificial metalloenzymes and provides a perspective on existing and future research in the area.