In vitro cultivation of Maritrema novaezealandensis (Microphallidae):: the effect of culture medium on excystation, survival and egg production
In vitro cultivation of Maritrema novaezealandensis (Microphallidae):: the effect of culture medium on excystation, survival and egg production
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DOI：
10.1007/s00436-004-1293-3
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发表时间：
2005-03-01
影响因子：
2
通讯作者：
Poulin, R
中科院分区：
文献类型：
--
作者：
Fredensborg, BL;Poulin, R
在本研究中，针对最近发现的小阴茎吸虫 Maritrema novaezealandensis，研究了培养基成分对排泄、生长、存活和产卵的影响。来自两个第二中间蟹宿主，粗眼大眼蟹和白色哈利蟹的囊蚴在以下任一种中孵育：(1) 0.85%盐水溶液，(2)商业细胞培养基NCTC-109，(3)补充有20%的NCTC-109，或(4)补充有40%鸡血清的NCTC-109。此外，将排出的囊蚴在三种培养基中分别培养5天：补充有20%或40%鸡血清的NCTC-109和NCTC-109。实验中使用的所有培养基均快速排出。然而，与其他培养基相比，0.85% 盐水溶液中的囊蚴在培养的前 4 小时内的排出率明显较低。在添加鸡血清的培养基中培养的标本中产蛋量最高，并在培养 2 天后达到最大值。然而，在三种培养基中的任何一种中培养第一天后都没有发生生长。
In this study, the effect of the composition of culture medium on excystation, growth, survival and egg production was investigated for the recently discovered microphallid trematode Maritrema novaezealandensis. Metacercariae from the two second intermediate crab hosts, Macrophthalmus hirtipes and Halicarcinus whitei, were incubated in either: (1) 0.85% saline solution, (2) the commercial cell culture medium, NCTC-109, (3) NCTC-109 supplemented with 20%, or (4) NCTC-109 supplemented with 40% chicken serum. Furthermore, excysted metacercariae were cultured for 5-days in each of the three media: NCTC-109 and NCTC-109 supplemented with 20% or 40% chicken serum. Excystment was rapid for all media used in the experiment. However, metacercariae in 0.85% saline solution had a significantly lower excystment rate over the first 4 h of incubation compared to the other media. Egg production was highest in specimens cultured in media with a supplement of chicken serum and reached a maximum after 2 days of cultivation. Growth, however, did not occur after the first day of cultivation in any of the three media.