Osteochondral repair with synovial membrane‑derived mesenchymal stem cells.
Osteochondral repair with synovial membrane‑derived mesenchymal stem cells.
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滑膜间充质干细胞修复骨软骨
DOI：
10.3892/mmr.2016.4795
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发表时间：
2016-03
影响因子：
3.4
通讯作者：
Chen S
中科院分区：
文献类型：
--
作者：
Li H;Qian J;Chen J;Zhong K;Chen S
本研究的目的是分析滑膜源间充质干细胞（SMSC）移植后兔骨软骨缺损的软骨修复组织质量。选取15只新西兰大白兔，随机分为3组，每组5只。在第1组中，在右膝滑车中建立骨软骨缺损模型，然后移植SMSC （SMSC组）。第2组不作进一步治疗，建立骨软骨缺损模型（对照组）。第三组不进行骨软骨缺损模型的建立，作为假对照（正常组）。手术后12周处死所有动物进行磁共振成像和组织学检查。对照组与SMSC组在宏观评分（P>0.05）、二维磁共振观察软骨修复组织评分（P>0.05）、改良O’driscoll评分（P>0.05）方面均无显著差异。与对照组相比，SMSCs组术后组织质量明显改善。与对照组相比，SMSCs组修复组织的T2较短，但差异无统计学意义（P < 0.05）。与对照组相比，SMSC组修复组织内表观扩散系数显著降低（P=0.016）。本研究结果表明，使用SMSCs进行骨软骨修复有助于修复适当的组织纹理。
The aim of the present study was to analyze cartilage repair tissue quality following synovial membrane-derived mesenchymal stem cell (SMSC) transplantation in a rabbit osteochondral defect. A total of 15 New Zealand white rabbits were randomly distributed into three groups (n=5 in each group). In group 1, an osteochondral defect model was established in the right knee trochlea, prior to transplantation with SMSCs (SMSC group). In group 2, an osteochondral defect model was established without further treatment (control group). Group 3 did not undergo osteochondral defect model establishment and served as the sham control (normal group). All animals were sacrificed 12 weeks following the surgical procedures for magnetic resonance imaging and histological examination. No significant differences were observed between the control and SMSC group in the macroscopic score (P>0.05), the 2D magnetic resonance observation of cartilage repair tissue score (P>0.05) or the modified O'Driscoll scale (P>0.05). Compared with the control group, a significant improvement in tissue quality was observed in the SMSCs group postoperatively. The repair tissue of the SMSCs group had a shorter T2, compared with that of the control group, although no significant difference was detected (P>0.05). Furthermore, the apparent diffusion coefficient in the repair tissue of the SMSC group had a significantly lower value, compared with that of the control group (P=0.016). The results of the present study demonstrated that osteochondral repair using SMSCs facilitated the repair of appropriate tissue texture.