Widespread Occurrence of Diverse Human Pathogenic Types of the Fungus Fusarium Detected in Plumbing Drains
Widespread Occurrence of Diverse Human Pathogenic Types of the Fungus Fusarium Detected in Plumbing Drains
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DOI：
10.1128/jcm.05468-11
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发表时间：
2011-12-01
影响因子：
9.4
通讯作者：
Geiser, David M.
中科院分区：
文献类型：
--
作者：
Short, Dylan P. G.;O'Donnell, Kerry;Geiser, David M.
有人提出，管道系统可能是人类致病性镰刀菌分离株的重要环境储存库。我们测试了这一假设，进行了第一次广泛的多位点序列分型（MLST）调查的管道排水相关的镰刀菌分离株，并比较观察到的多样性的临床镰刀菌分离株的已知多样性。我们使用擦洗方法从美国131栋建筑物中抽取了471个排水管，其中大部分是浴室水槽。我们发现，接受调查的66%的水槽和80%的建筑物至少产生了一种镰刀菌培养物。共收集了297株镰刀菌进行MLST，以确定这些菌株的系统发育物种和序列类型（ST）。我们的调查显示，水槽中六种最常见的ST与六种最常与人类感染相关的ST相同。我们推测，最普遍的ST，凭借他们的能力，形成和生长在生物膜，很好地适应管道系统。六个主要的镰刀菌ST经常从一个广泛的地理区域内的管道排水系统中分离出来，并且与经常与人类感染相关的ST相同。
It has been proposed that plumbing systems might serve as a significant environmental reservoir of human-pathogenic isolates of Fusarium. We tested this hypothesis by performing the first extensive multilocus sequence typing (MLST) survey of plumbing drain-associated Fusarium isolates and comparing the diversity observed to the known diversity of clinical Fusarium isolates. We sampled 471 drains, mostly in bathroom sinks, from 131 buildings in the United States using a swabbing method. We found that 66% of sinks and 80% of buildings surveyed yielded at least one Fusarium culture. A total of 297 isolates of Fusarium collected were subjected to MLST to identify the phylogenetic species and sequence types (STs) of these isolates. Our survey revealed that the six most common STs in sinks were identical to the six most frequently associated with human infections. We speculate that the most prevalent STs, by virtue of their ability to form and grow in biofilms, are well adapted to plumbing systems. Six major Fusarium STs were frequently isolated from plumbing drains within a broad geographic area and were identical to STs frequently associated with human infections.