Characterization of the polycystic kidney disease 2 gene promoter.
Characterization of the polycystic kidney disease 2 gene promoter.
复制标题
DOI：
10.1016/j.ygeno.2014.08.006
复制
发表时间：
2014-12
期刊：
影响因子：
4.4
通讯作者：
Qingsong Wang;Gang Han;Jianhua Ye;Xianjun Gao;Hongjing Niu;Jingwen Zhao;Yawen Chai;Ning Li;H. Yin
中科院分区：
文献类型：
--
作者：
Qingsong Wang;Gang Han;Jianhua Ye;Xianjun Gao;Hongjing Niu;Jingwen Zhao;Yawen Chai;Ning Li;H. Yin
PKD 2转录的关键调控元件尚不清楚。为了进一步确定这些核心元件，我们利用生物信息学和分子生物学手段对猪PKD 2启动子进行了分析，发现猪PKD 2启动子具有典型的富含CpG和较少TATA的特征。进一步的研究表明，核心区域位于片段-483到+100。随后的生物物理结合分析和突变实验表明，G4基序和Sp1是介导猪PKD 2转录的关键调节因子。在PKD 2启动子区也有相同的调控模式，提示G4和Sp1在PKD 2的调控中起着重要作用。
The key regulatory elements forPKD2transcription remain unclear. To identify these core elements, we characterized porcinePKD2promoter with bioinformatics and molecular tools and found porcinePKD2promoter bearing typical features of enriched CpG and less TATA. Further studies demonstrated that the core region was located in fragment − 483 to + 100. Subsequent biophysical binding assays and mutation experiments revealed that G4 motif and Sp1 are critical regulators for mediating the transcription of porcinePKD2. Moreover, the same regulatory pattern was reproduced in humanPKD2promoter region, indicating the critical role of G4 and Sp1 in regulatingPKD2.