THE 3-DIMENSIONAL ORGANIZATION OF PLASMALEMMAL VESICULAR PROFILES IN THE ENDOTHELIUM OF RAT-HEART CAPILLARIES
THE 3-DIMENSIONAL ORGANIZATION OF PLASMALEMMAL VESICULAR PROFILES IN THE ENDOTHELIUM OF RAT-HEART CAPILLARIES
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DOI：
10.1016/0026-2862(83)90025-0
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发表时间：
1983-01-01
影响因子：
3.1
通讯作者：
CRONE, C
中科院分区：
文献类型：
--
作者：
BUNDGAARD, M;HAGMAN, P;CRONE, C
本文对大鼠心脏毛细血管内皮细胞质膜泡的结构进行了再研究。从随机薄切片判断，50%的囊泡游离在细胞质中，其余的向内皮细胞表面开放，这一分布与先前的研究一致。三维重建的基础上连续切片（厚度. simeq。12毫米）给出了一个非常不同的画面。所有质膜泡状配置文件（921从5毛细管）的表面膜的一部分，无论是作为小窝或更复杂的总状内陷。这种组织以前曾在青蛙肠系膜毛细血管中观察到。毛细血管内皮细胞的一个普遍特征显然是游离质膜小泡的缺乏或极少。因此，术语内皮细胞质膜囊泡应替换为内皮细胞质膜内陷。显然，跨内皮囊泡运输是不太可能发生的，这一膜系统执行其他，迄今未知，funcitons。
The organization of plasmalemmal vesicular profiles in the endothelium of rat heart capillaries was reinvestigated. Judged from random thin sections, .apprx. 50% of the vesicles appeared free in the cytoplasm, the rest opening to the surfaces of the endothelial cells, a distribution which coroborates previous studies. Three-dimensional reconstructions based on ultrathin serial sections (thickness .simeq. 12 mm) gave a very different picture. All plasmalemmal vesicular profiles (921 from 5 capillaries) were parts of the surface membrane either as caveolae or as more complex racemose invaginations. This organization has previously been observed in frog mesenteric capillaries. Absence or extreme rarity of free plasmalemma vesicles apparently is a general feature of capillary endothelia. Consequently, the term endothelial, plasmalemmal vesicles should be replaced by endothelial plasmalemmal invaginations. Apparently, transendothelial vesicular transport is unlikely to occur; this membrane system performs other, as yet unknown, funcitons.