DNA of human cytomegalovirus: size heterogeneity and defectiveness resulting from serial undiluted passage
DNA of human cytomegalovirus: size heterogeneity and defectiveness resulting from serial undiluted passage
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人巨细胞病毒 DNA：连续未稀释传代导致的大小异质性和缺陷
DOI：
10.1128/jvi.31.1.231-239.1979
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发表时间：
1979
影响因子：
5.4
通讯作者：
D. Thomsen
中科院分区：
文献类型：
--
作者：
M. Stinski;E. Mocarski;D. Thomsen
与噬斑纯化、低多重传代人巨细胞病毒（Towne株）相关的大多数DNA分子的分子量约为150 x 10（6），分子复杂性约为140 x 10（6）。连续高多重性传代导致产生缺陷性巨细胞病毒。包装成病毒体的较小DNA分子的积累与感染性的降低和颗粒与PFU比率的增加直接相关。大多数缺陷DNA分子的分子量约为100 × 10（6）。此外，还有一些病毒DNA分子约为60 × 10（6）。限制性内切酶分析缺陷的巨细胞病毒DNA检测到独特的DNA片段，表明在核苷酸序列的特定改变。一些原因产生的缺陷巨细胞病毒DNA进行了讨论。
The majority of DNA molecules associated with plaque-purified, low-multiplicity-passaged human cytomegalovirus (Towne strain) had a molecular weight of approximately 150 x 10(6) and a molecular complexity of approximately 140 x 10(6). Serial high-multiplicity passage resulted in the production of defective cytomegalovirions. An accumulation of smaller DNA molecules packaged into virions was directly correlated with a decrease in infectivity and an increase in the particle-to-PFU ratio. The majority of defective DNA molecules had a molecular weight of approximately 100 x 10(6). In addition, there were some viral DNA molecules of approximately 60 x 10(6). Restriction enzyme analysis of defective cytomegalovirus DNA detected unique DNA fragments, suggesting a specific alteration in the nucleotide sequence. Some reasons for the generation of defective cytomegalovirus DNA are discussed.