Intestine-Specific Delivery of Hydrophobic Bioactives from Oxidized Starch Microspheres with an Enhanced Stability
Intestine-Specific Delivery of Hydrophobic Bioactives from Oxidized Starch Microspheres with an Enhanced Stability
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氧化淀粉微球疏水性生物活性物质的肠道特异性递送，稳定性增强
DOI：
10.1021/acs.jafc.5b03575
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发表时间：
2015
影响因子：
6.1
通讯作者：
Li Yuan
中科院分区：
文献类型：
--
作者：
Wang Shanshan;Chen Yuying;Lang Hao;Chen Yiming;Shi Mengxuan;Wu Ji;e;Liu Xianwu;Li Zuseng;Liu Bin;Yuan Qipeng;Li Yuan
开发了一种具有改善稳定性的疏水生物活性物质的肠道特异性递送系统。它由氧化马铃薯淀粉聚合物组成，其中羧基通过铁离子物理交联。模型疏水成分（β-胡萝卜素）通过双乳液法掺入淀粉微球内。激光共聚焦扫描显微镜图像显示，β-胡萝卜素均匀分布在淀粉微球的内油相中。微球的ζ电位的负值随着pH的增大和离子强度的减小而增大。体外释放实验表明，微球在酸性胃条件下（pH < 2）是稳定的，而在中性肠道条件下（pH 7.0），微球破裂释放负载的β-胡萝卜素。对1,1-二苯基-2-吡咯酰肼基自由基2,2-二苯基-1-（2,4,6-三硝基苯）的清除活性结果表明，微球包封的β-胡萝卜素在80℃热处理后具有较好的清除活性。包封后的β-胡萝卜素在室温下的贮藏稳定性也得到了提高。淀粉微球显示出作为肠道特异性载体的潜力，具有增强的稳定性。
An intestine-specific delivery system for hydrophobic bioactives with improved stability was developed. It consists of oxidized potato starch polymers, where the carboxyl groups were physically cross-linked via ferric ions. The model hydrophobic ingredients (β-carotene) were incorporated inside the starch microspheres via a double-emulsion method. Confocal laser scanning microscopy images showed that β-carotene were distributed homogeneously in the inner oil phase of the starch microspheres. The negative value of the ζ-potential of microspheres increased with increasing pH and decreasing ionic strength.In vitrorelease experiments showed that the microspheres were stable at acidic stomach conditions (pH < 2), whereas at neutral intestinal conditions (pH 7.0), they rupture to release the loaded β-carotene. The 1,1-diphenyl-2-picrylhydrazyl radical, 2,2-diphenyl-1-(2,4,6-trinitriphenyl), scavenging activity results suggested that microsphere-encapsulated β-carotene had an improved activity after thermal treatment at 80 °C. The storage stability of encapsulated β-carotene at room temperature was also enhanced. The starch microspheres showed potential as intestine-specific carriers with an enhanced stability.