A dynamically deformable microfilter for selective separation of specific substances in microfluidics
A dynamically deformable microfilter for selective separation of specific substances in microfluidics
复制标题
DOI：
10.1063/5.0025927
复制
发表时间：
2020-11-01
期刊：
影响因子：
3.2
通讯作者：
Nakashima, Yuta
中科院分区：
文献类型：
--
作者：
Kumamoto, Seitaro;Nakatake, Kenshiro;Nakashima, Yuta
为了研究环境或生物溶液，必须分离其组分。在这项研究中，一个三维可变形的动态微过滤器的开发，选择性地从溶液中分离的目标物质。这种微滤波器是一种精细的金属镍结构，采用光刻和电镀技术制造。它的整个表面镀有金，并有多个10-20 μ m宽的狭缝。当用于流体分离时，由于流体动力，其二维形状变形为三维形状。通过调节施加到微过滤器的压力，可以改变由变形产生的差距的尺寸。为了有效地分离目标物质，研究了溶液流速与微过滤器变形程度之间的关系。过滤实验证明了微过滤器在弹性变形的情况下分离目标物质而不经历塑性变形的能力。此外，用核酸适体修饰微滤器表面导致靶细胞的选择性分离，这进一步证明了微滤器在分离异质溶液的特定组分中的潜在应用。
To study an environmental or biological solution, it is essential to separate its constituents. In this study, a 3D-deformable dynamic microfilter was developed to selectively separate the target substance from a solution. This microfilter is a fine metallic nickel structure fabricated using photolithography and electroplating techniques. It is gold-coated across its entire surface with multiple slits of 10-20 mu m in width. Its two-dimensional shape is deformed into a three-dimensional shape when used for fluid separation due to hydrodynamic forces. By adjusting the pressure applied to the microfilter, the size of the gap created by deformation can be changed. To effectively isolate the target substance, the relationship between the solution flow rate and the extent of microfilter deformation was investigated. The filtration experiments demonstrated the microfilter's ability to isolate the target substance with elastic deformation without undergoing plastic deformation. Additionally, modification of the microfilter surface with nucleic acid aptamers resulted in the selective isolation of the target cell, which further demonstrates the potential application of microfilters in the isolation of specific components of heterogeneous solutions.