Two Parallel Pathways Assign Opposing Odor Valences during Drosophila Memory Formation
Two Parallel Pathways Assign Opposing Odor Valences during Drosophila Memory Formation
复制标题
果蝇记忆形成过程中两条平行途径分配相反的气味价
DOI：
10.1016/j.celrep.2018.02.012
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发表时间：
2018
期刊：
影响因子：
8.8
通讯作者：
Tabata Tetsuya
中科院分区：
文献类型：
--
作者：
Yamazaki Daisuke;Hiroi Makoto;Abe Takashi;Shimizu Kazumichi;Minami-Ohtsubo Maki;Maeyama Yuko;Horiuchi Junjiro;Tabata Tetsuya
在果蝇的嗅觉联想学习过程中，气味激活了特定的固有蘑菇体（MB）神经元亚群。同时暴露于奖励或惩罚被认为激活外源性多巴胺能神经元，其调节气味编码MB神经元和MB输出神经元之间的突触连接以改变行为。然而，在这里，我们根据cAMP反应元件（CRE）依赖性表达，γCRE-p和γCRE-n，编码厌恶和食欲价，确定了两类内在MB γ神经元。γCRE-p和γCRE-n神经元拮抗作用，以维持中性气味的中性价。任何一种细胞类型的激活或抑制都会扰乱这种平衡，将气味偏好切换到正值或负值。蘑菇体输出神经元MBON-γ5β′2a/β′ 2 mp和MBON-γ2α′1介导γCRE-p和γCRE-n神经元的活动。我们的数据表明，MB神经元编码价信息，以及气味信息，这些信息是通过一个涉及MBON的过程来调节学习和记忆。
During olfactory associative learning inDrosophila, odors activate specific subsets of intrinsic mushroom body (MB) neurons. Coincident exposure to either rewards or punishments is thought to activate extrinsic dopaminergic neurons, which modulate synaptic connections between odor-encoding MB neurons and MB output neurons to alter behaviors. However, here we identify two classes of intrinsic MB γ neurons based on cAMP response element (CRE)-dependent expression, γCRE-p and γCRE-n, which encode aversive and appetitive valences. γCRE-p and γCRE-n neurons act antagonistically to maintain neutral valences for neutral odors. Activation or inhibition of either cell type upsets this balance, toggling odor preferences to either positive or negative values. The mushroom body output neurons, MBON-γ5β′2a/β′2mp and MBON-γ2α′1, mediate the actions of γCRE-p and γCRE-n neurons. Our data indicate that MB neurons encode valence information, as well as odor information, and this information is integrated through a process involving MBONs to regulate learning and memory.