Pneumococcus with the "6E" cps Locus Produces Serotype 6B Capsular Polysaccharide
Pneumococcus with the "6E" cps Locus Produces Serotype 6B Capsular Polysaccharide
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DOI：
10.1128/jcm.03194-15
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发表时间：
2016-04-01
影响因子：
9.4
通讯作者：
Nahm, Moon H.
中科院分区：
文献类型：
--
作者：
Burton, Robert L.;Geno, K. Aaron;Nahm, Moon H.
对血清组6肺炎链球菌分离株的遗传研究确定了推定的血清型6E。尽管其荚膜多糖结构尚未阐明，但在越来越多的研究中，推定的血清型6E被描述为潜在的新血清型。我们在这里表明，SPEC6B，这是广泛使用的血清型6B调理吞噬试验的靶菌株，具有推定的血清型6E的遗传特征，但产生的荚膜多糖相同的6B荚膜多糖，确定由一维（1D）和二维核磁共振（NMR）。因此，推定的血清型6E仅仅是血清型6B的遗传变体。此外，SPEC6B适合作为血清型6B调理吞噬试验的靶菌株。这个例子说明了仅根据遗传发现来分配新的细菌血清型的困难。
Genetic studies of serogroup 6 isolates of Streptococcus pneumoniae identified putative serotype 6E. Although its capsular polysaccharide structure has not been elucidated, putative serotype 6E is described in an increasing number of studies as a potentially new serotype. We show here that SPEC6B, which is widely used as a target strain for serotype 6B opsonophagocytosis assays, has the genetic features of the putative serotype 6E but produces capsular polysaccharide identical to 6B capsular polysaccharide as determined by one-dimensional (1D) and 2D nuclear magnetic resonance (NMR). Thus, putative serotype 6E is a mere genetic variant of serotype 6B. Also, SPEC6B is appropriate as a target strain for serotype 6B opsonophagocytosis assays. This example illustrates the difficulties of assigning new bacterial serotypes based on genetic findings alone.