Roles of GPR41 and GPR43 in leptin secretory responses of murine adipocytes to short chain fatty acids
Roles of GPR41 and GPR43 in leptin secretory responses of murine adipocytes to short chain fatty acids
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DOI：
10.1016/j.febslet.2010.04.027
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发表时间：
2010-06-03
期刊：
影响因子：
3.5
通讯作者：
Arch, Jonathan R. S.
中科院分区：
文献类型：
--
作者：
Zaibi, Mohamed S.;Stocker, Claire J.;Arch, Jonathan R. S.
据报道，GPR41在鼠脂肪组织中表达，并通过激活G α（i）介导短链脂肪酸（SCFA）刺激的瘦素分泌。在这里，我们同意一个矛盾的报告，发现没有表达的GPR41在小鼠脂肪组织。然而，在腺苷脱氨酶存在的情况下，通过腺苷A1受体使G α（i）信号最小化，SCFA刺激野生型而非GPR41敲除小鼠脂肪细胞的瘦素分泌。在GPR41敲除小鼠中GPR43的表达降低。乙酸盐而不是丁酸盐刺激野生型肠系膜脂肪细胞中的瘦素分泌，这与GPR43而不是GPR41介导的应答一致。百日咳毒素阻止了丙酸盐对附睾脂肪细胞瘦素分泌的刺激，这表明GPR43介导的G alpha（i）信号传导参与了SCFA刺激的瘦素分泌。(C)2010年欧洲生物化学学会联合会。由Elsevier B出版。V.保留所有权利。
GPR41 is reportedly expressed in murine adipose tissue and mediates short chain fatty acid (SCFA)-stimulated leptin secretion by activating G alpha(i). Here, we agree with a contradictory report in finding no expression of GPR41 in murine adipose tissue. Nevertheless, in the presence of adenosine deaminase to minimise G alpha(i) signalling via the adenosine A1 receptor, SCFA stimulated leptin secretion by adipocytes from wild-type but not GPR41 knockout mice. Expression of GPR43 was reduced in GPR41 knockout mice. Acetate but not butyrate stimulated leptin secretion in wild-type mesenteric adipocytes, consistent with mediation of the response by GPR43 rather than GPR41. Pertussis toxin prevented stimulation of leptin secretion by propionate in epididymal adipocytes, implicating G alpha(i) signalling mediated by GPR43 in SCFA-stimulated leptin secretion. (C) 2010 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.