Evaluation of enzyme activities by gas chromatography-mass spectrometry: HMGCoA reductase and cholesterol 7 alpha-hydroxylase.
Evaluation of enzyme activities by gas chromatography-mass spectrometry: HMGCoA reductase and cholesterol 7 alpha-hydroxylase.
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通过气相色谱-质谱法评估酶活性：HMGCoA 还原酶和胆固醇 7 α-羟化酶。
DOI：
10.1016/s0021-9673(00)95094-5
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发表时间：
1984
期刊：
影响因子：
--
通讯作者：
R. Paoletti
中科院分区：
文献类型：
--
作者：
M. Galli Kienle;G. Galli;E. Bosisio;G. Cighetti;R. Paoletti
描述了用于评价HMGCoA还原酶和胆固醇7α-羟化酶活性的方法。该方法分别基于通过选择离子监测HMGCoA形成的甲羟戊酸和胆固醇形成的7α-羟基胆固醇的测定。该方法与基于使用放射性同位素的方法一样灵敏，但耗时更少，因为对总脂质提取物进行定量。对于胆固醇7α-羟化酶，该方法不需要使用外源性胆固醇作为底物，因此避免了与内源性微粒体胆固醇平衡相关的问题。
Methods are described for the evaluation of HMGCoA reductase and cholesterol 7α-hydroxylase activities. The methods are based on the measurement by selected-ion monitoring of mevalonate, formed from HMGCoA and of 7α-hydroxycholesterol formed from cholesterol, respectively. The methods are as sensitive as those based on the use of radioisotopes but less time-consuming because quantitation is carried out on total lipid extracts. In the case of cholesterol 7α-hydroxylase, the procedure does not require the use of exogenous cholesterol as the substrate, so problems related to its equilibration with endogenous microsomal cholesterol are avoided.