Association of cytokine polymorphic inheritance and in vitro cytokine production in anti-CD3/CD28-stimulated peripheral blood lymphocytes
Association of cytokine polymorphic inheritance and in vitro cytokine production in anti-CD3/CD28-stimulated peripheral blood lymphocytes
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DOI：
10.1097/00007890-200110270-00019
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发表时间：
2001-10-27
期刊：
影响因子：
6.2
通讯作者：
Blair, PJ
中科院分区：
文献类型：
--
作者：
Hoffmann, SC;Stanley, EM;Blair, PJ
背景。细胞因子基因的遗传变异被认为调节细胞因子蛋白的产生。然而，使用T细胞有丝分裂原的研究并不总是证明细胞因子多态性与体外蛋白质生产之间的显着关系。此外，IL-2基因-330位点多态性的功能后果尚未被描述。我们将体外培养外周血淋巴细胞共刺激后的蛋白生成与细胞因子基因多态性基因型联系起来。从40名健康志愿者中分离PBL。采用酶联免疫吸附法测定细胞因子蛋白的产生。采用聚合酶链式反应（PCR）检测白细胞介素- (IL) 2、IL-6、IL-10、肿瘤坏死因子（tnf - α）、肿瘤生长因子（tgf - β）和干扰素（ifn - γ）的多态性。在48小时的刀豆蛋白a刺激后，IL-10、ifn - γ和tnf - α基因的蛋白生成和细胞因子多态性变异的统计学差异不明显。相比之下，抗cd3 /CD28刺激后差异有统计学意义(P
Background. Genetic variations in cytokine genes are thought to regulate cytokine protein production. However, studies using T cell mitogens have not always demonstrated a significant relationship between cytokine polymorphisms and in vitro protein production. Furthermore, the functional consequence of a polymorphism at position -330 in the IL-2 gene has not been described. We associated in vitro protein production with cytokine gene polymorphic genotypes after costimulation of cultured peripheral blood lymphocytes.Methods. PBL were isolated from forty healthy volunteers. Cytokine protein production was assessed by enzyme-linked immunosorbent assay. Polymorphisms in interleukin- (IL) 2, IL-6, IL-10, tumor necrosis factor (TNF-alpha), tumor growth factor (TGF-beta), and interferon (IFN-gamma) were determined by polymerase chain reaction (PCR).Results. Statistical difference between protein production and cytokine polymorphic variants in the IL-10, IFN-gamma, and TNF-alpha genes was not evident after 48-hour stimulation with concanavalin-A. In contrast, after anti-CD3/CD28 stimulation significant differences (P