Convergent premotoneuronal inputs to single trigeminal motoneurons.
Convergent premotoneuronal inputs to single trigeminal motoneurons.
复制标题
将前运动神经元输入汇聚到单个三叉神经运动神经元。
DOI：
10.1177/0022034512453724
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发表时间：
2012
期刊：
影响因子：
7.6
通讯作者：
Iijima T and Inoue T.
中科院分区：
文献类型：
--
作者：
Nonaka M;Nishimura A;Nakamura S;Nakayama K;Mochizuki A;Iijima T and Inoue T.
由于以三叉神经运动神经元为靶点的运动前神经元分布在不同的区域，包括三叉神经上核(SUV)和三叉神经间核(INV)、三叉神经主感觉核(PRV)和PRV背侧区(DRT)，单个三叉神经运动神经元可能从这些区域接受不同的汇聚输入。因此，我们用全细胞记录和笼养谷氨酸激光光解的方法，研究了来自这些区域的突触传入到P1-5新生大鼠脑干切片中的咬肌运动神经元(MMN)和二腹运动神经元(DMNS)的特性。光刺激SUV、INV、PRV和DRT内的多个区域在19个MMN中的14个和26个DMN中的18个诱发突触后电流(PSCs)。此外，刺激外侧上丘脑的爆发式PSCs在MMN比低频PSCs在MMN或在DMNS更常见。在GABA受体拮抗剂SR95531和甘氨酸受体拮抗剂士的宁的存在下，也得到了类似的结果。这些结果表明，新生MMN和DMNS都接受来自SUV、INTV、PRV和DRT的汇聚性谷氨酸能输入，而外侧SUV主要向MMN发送突发性输入。这种向三叉神经运动神经元的会聚前运动神经元输入可能有助于新生儿或运动功能的正确执行。
Because pre-motor neurons targeting trigeminal motoneurons are located in various regions, including the supratrigeminal (SupV) and intertrigeminal (IntV) regions, the principal sensory trigeminal nucleus (PrV), and the region dorsal to the PrV (dRt), a single trigeminal motoneuron may receive differential convergent inputs from these regions. We thus examined the properties of synaptic inputs from these regions to masseter motoneurons (MMNs) and digastric motoneurons (DMNs) in brainstem slice preparations obtained from P1-5 neonatal rats, using whole-cell recordings and laser photolysis of caged glutamate. Photostimulation of multiple regions within the SupV, IntV, PrV, and dRt induced post-synaptic currents (PSCs) in 14 of 19 MMNs and 18 of 26 DMNs. Furthermore, the stimulation of the lateral SupV significantly induced burst PSCs in MMNs more often than low-frequency PSCs in MMNs or burst PSCs in DMNs. Similar results were obtained in the presence of the GABAAreceptor antagonist SR95531 and the glycine receptor antagonist strychnine. These results suggest that both neonatal MMNs and DMNs receive convergent glutamatergic inputs from the SupV, IntV, PrV, and dRt, and that the lateral SupV sends burst inputs predominantly to the MMNs. Such convergent pre-motoneuronal inputs to trigeminal motoneurons may contribute to the proper execution of neonatal oro-motor functions.