Screening of plantaricin EF and JK in an Algerian Lactobacillus plantarum isolate.
Screening of plantaricin EF and JK in an Algerian Lactobacillus plantarum isolate.
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DOI：
10.1016/s2222-1808(15)60819-2
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发表时间：
2015-01-01
期刊：
影响因子：
--
通讯作者：
Thonart, P.
中科院分区：
文献类型：
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作者：
Chaalel, A.;Riazi, A.;Thonart, P.
目的：从健康婴儿粪便中分离一株拮抗乳酸菌菌株，并评估其对一系列革兰氏阳性和革兰氏阴性细菌的拮抗能力。方法：采用生化试验和16S rDNA序列分离。利用特异引物对植物皂苷细菌素编码基因进行PCR筛选。用琼脂斑点法和琼脂孔扩散法测定其抑菌活性。对废培养上清进行超滤。考察了丝裂霉素C对紫外光、热处理、贮藏、pH和酶的影响。结果：生化检测和16S rDNA序列显示LbM2a属于植物乳杆菌。植物素基因筛选显示存在植物素EF和K.超滤测试导致估计分子量在3和10 kDa之间。LbM2a表现出广泛的抑制活性，在pH 2.0 ~ 6.0范围内稳定，且具有蛋白性（被蛋白水解酶失活）。丝裂霉素C和紫外光对其活性没有影响。结论：该分离物及其细菌素对多种细菌的抑制作用对食品安全具有重要意义，在食品防腐剂方面具有广阔的应用前景。
Objective: To isolate an antagonistic Lactobacillus strain from healthy infant feces and estimate its potential against a range of Gram-positive and Gram-negative bacteria. Methods: Isolation was based on biochemical tests and on 16S rDNA sequences. A PCR based screening of plantaricin bacteriocin encoding genes was conducted using specific primers. Antimicrobial activity was realized using spot on agar and agar well-diffusion tests. Spent culture supernatant was subjected to ultrafiltration. Effect of mitomycin C and UV light, heat treatment, storage, pH and enzymes were tested. Results: Biochemical tests and 16S rDNA sequences showed that LbM2a belonged to Lactobacillus plantarum. Screening of plantaricin genes showed the presence of plantaricin EF and K. Ultrafiltration tests lead to estimate the molecular weight between 3 and 10 kDa. LbM2a showed a broad inhibitory activity, which was stable at pH range of 2.0 to 6.0 and was proteinaceous (inactivation by proteolytic enzymes). Mitomycin C and UV light did not affect the activity. Conclusions: The paper illustrates that the ability of isolate and its bacteriocins in inhibiting a wide-range of bacteria are great interest for food safety and might have future applications as food preservative.