FACTORS AFFECTING THE DILUTION, FREEZING AND STORAGE OF HUMAN SEMEN
FACTORS AFFECTING THE DILUTION, FREEZING AND STORAGE OF HUMAN SEMEN
复制标题
影响人类精液稀释、冷冻和储存的因素
DOI：
10.1530/jrf.0.0110001
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发表时间：
1966
期刊：
影响因子：
3.8
通讯作者：
J. Wiederman
中科院分区：
文献类型：
--
作者：
M. Freund;J. Wiederman
与某些稀释剂的毒性作用相反，人类精液用六种不同稀释剂以1：1、1：5、1：25和1：125稀释的实验表明存在真正的“稀释效应”。Norman-Johnson-溶液1和2优于所有其他供试稀释剂，并且在冷冻稀释的人精液时作为培养基同样有用。使用发展到这一点的技术进行了21次冷冻试验：用Norman-Johnson溶液1或2稀释，加入10%蛋黄，在4小时内从+25 ℃缓慢冷却至+5 ℃，在+5 ℃下用7%甘油平衡6小时，以-1\s = deg\C/min的速率从+5到-70\s = deg\C缓慢冷冻，并在-1\s = deg\C/min的温度下储存
The presence of a true `dilution effect', as opposed to the toxic effects of certain diluents, was indicated in experiments where human semen was diluted 1:1, 1:5, 1 : 25 and 1 : 125 with six different diluents. Norman-Johnson-Solutions 1 and 2 were found to be superior to all other diluents tested and were shown to be equally useful as media in the freezing of diluted human semen. Twenty-one freezing trials were made, using the technique as developed to this point\p=m-\ 1 : 5 dilution with Norman-Johnson-Solutions 1 or 2, the addition of 10% egg yolk, slow cooling from +25 to +5\s=deg\C over a 4-hr period, 6 hr of equilibration with 7% glycerol at +5\s=deg\C, slow freezing from +5 to -70\s=deg\C at the rate of -1\s=deg\C/min, and storage at temperatures of