Antimicrobial Potential of Lycosin-I, a Cationic and Amphiphilic Peptide from the Venom of the Spider Lycosa singorensis
Antimicrobial Potential of Lycosin-I, a Cationic and Amphiphilic Peptide from the Venom of the Spider Lycosa singorensis
复制标题
Lycosin-I 的抗菌潜力，一种来自 Lycosa Singorensis 蜘蛛毒液的阳离子和两亲性肽
DOI：
10.2174/15665240113139990045
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发表时间：
2013-07-01
影响因子：
2.5
通讯作者：
Liang, S.
中科院分区：
文献类型：
--
作者：
Tan, H.;Ding, X.;Liang, S.
抗菌肽是天然免疫系统的重要组成部分，在抵抗细菌感染中起着不可或缺的作用。在这里，我们研究了石蒜素-I的抗菌活性，石蒜素-I是一种24个残基的阳离子抗癌肽，来自Lycosa singorensis，与几种阳离子和两亲性抗菌肽具有高度的结构相似性。用微量稀释法测定了石蒜素-I对27种微生物（包括细菌和真菌）的抗微生物活性，并与Xenopus衍生的AMP magainin 2进行了比较。Lycosin-I在低微摩尔浓度下抑制大多数微生物的生长，并且是比爪蟾抗菌肽2更有效的抑制剂。石蒜素-I具有快速、选择性、广谱的杀菌活性，并与传统抗生素有协同作用。在体内，它在小鼠大腿感染模型中显示出有效的杀菌活性。高Mg ~（2+）浓度降低了石蒜素-I的抗菌作用，这意味着该肽可能直接与细菌细胞膜相互作用。通过扫描电子显微镜观察到的荧光染料SYTOX的摄取和石蒜素-I处理的细菌细胞表面的变化证实，石蒜素-I透化细胞膜，导致快速杀菌效果。总之，我们的研究结果表明，石蒜素-I是一个有前途的肽与潜在的开发新的抗菌剂。
Antimicrobial peptides (AMPs) are significant components of the innate immune system and play indispensable roles in the resistance to bacterial infection. Here, we investigated the antimicrobial activity of lycosin-I, a 24-residue cationic anticancer peptide derived from Lycosa singorensis with high structural similarity to several cationic and amphiphilic antimicrobial peptides. The antimicrobial activity of lycosin-I against 27 strains of microbes including bacteria and fungi was examined and compared with that of the Xenopus-derived AMP magainin 2 using a microdilution assay. Lycosin-I inhibited the growth of most microorganisms at low micromolar concentrations, and was a more potent inhibitor than magainin 2. Lycosin-I showed rapid, selective and broad-spectrum bactericidal activity and a synergistic effect with traditional antibiotics. In vivo, it showed potent bactericidal activity in a mouse thigh infection model. High Mg2+ concentrations reduced the antibacterial effect of lycosin-I, implying that the peptide might directly interact with the bacterial cell membrane. Uptake of the fluorogenic dye SYTOX and changes in the surface of lycosin-I-treated bacterial cells observed by scanning electron microscopy confirmed that lycosin-I permeabilized the cell membrane, resulting in the rapid bactericidal effect. Taken together, our findings indicate that lycosin-I is a promising peptide with the potential for the development of novel antibacterial agents.