Graphene Platform for Hairpin-DNA-Based Impedimetric Genosensing
Graphene Platform for Hairpin-DNA-Based Impedimetric Genosensing
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DOI：
10.1021/nn200091p
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发表时间：
2011-03-01
期刊：
影响因子：
17.1
通讯作者：
Pumera, Martin
中科院分区：
文献类型：
--
作者：
Bonanni, Alessandra;Pumera, Martin
存在对DNA链的单核苷酸多态性的灵敏和选择性检测的巨大需求，因为该问题与许多主要疾病和病症（例如帕金森病和阿尔茨海默病）相关。为了实现检测的灵敏度和选择性，需要具有高表面积的信号的高灵敏度换能器。在这项工作中，我们采用了石墨烯平台联合收割机结合电化学阻抗谱的灵敏度与发夹形DNA探针的高选择性，用于快速检测与阿尔茨海默病的发展相关的单核苷酸多态性。我们研究了由不同数量的相同大小的石墨烯层组成的各种石墨烯平台的影响。我们相信，我们的发现是迈向高度敏感和选择性传感架构的重要一步。
There is enormous need for sensitive and selective detection of single nucleotide polymorphism of a DNA strand as this issue is related to many major diseases and disorders, such as Parkinson's and Alzheimer's disease. To achieve sensitivity and selectivity of the detection, a highly sensitive transducer of the signal with high surface area is required. In this work we employ a graphene platform to combine the sensitivity of electrochemical Impedance spectroscopy with the high selectivity of hairpin shaped DNA probes for the rapid detection of single nucleotide polymorphism correlated to the development of Alzheimer's disease. We investigate the influence of various graphene platforms consisting of different numbers of same sized graphene layers. We believe that our findings are an important step toward highly sensitive and selective sensing architectures.