Isolation and characterization of microsatellite loci in the intertidal snail Batillaria multiformis (Mollusca Gastropoda)
Isolation and characterization of microsatellite loci in the intertidal snail Batillaria multiformis (Mollusca Gastropoda)
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潮间带蜗牛 Batillaria multiformis（软体动物腹足纲）微卫星位点的分离和表征
DOI：
10.1007/s12686-013-0046-y
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发表时间：
2014
影响因子：
1.1
通讯作者：
K. Hirose and S. Kojima
中科院分区：
文献类型：
--
作者：
Itoh;H.;S. Kamimura;K. Hirose and S. Kojima
潮间带蜗牛属是日本各地潮间带常见的一种蜗牛。近年来，由于沿海地区的填海工程，多形小檗的种群数量有所减少。利用下一代测序技术，我们开发了11个微卫星标记来揭示群体间的遗传多样性和基因流动，并将它们组合成一个单一的多重面板。结果表明，Mangokuura河口和Kasari湾两个种群的等位基因数量分别为2 ~ 17个和2 ~ 15个，期望杂合度分别为0.274 ~ 0.936和0.455 ~ 0.934。
Intertidal snails of the genusBatillariaare a common group in tidelands throughout Japan. Recently, population sizes ofBatillaria multiformishave been reduced due to coastal area reclamation. Using next generation sequencing, we developed 11 microsatellite markers to reveal the genetic diversity and gene flow among populations and combined them into a single multiplex panel. Evaluating polymorphisms in two populations showed that the numbers of alleles per locus ranged from 2 to 17 and 2 to 15, and the expected heterozygosity ranged from 0.274 to 0.936 and 0.455 to 0.934 in Mangokuura estuary and Kasari Bay, respectively.