Cancer borealis stomatogastric nervous system dissection.
Cancer borealis stomatogastric nervous system dissection.
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DOI：
10.3791/1207
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发表时间：
2009-03-23
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影响因子：
--
通讯作者：
Grashow, Rachel G
中科院分区：
文献类型：
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作者：
Gutierrez, Gabrielle J;Grashow, Rachel G
口胃神经节（STG）是研究细胞和网络相互作用的一个很好的模型，因为它包含相对较少的细胞（大约25个），这些细胞被很好地表征。STG中的细胞表现出广泛的输出，并负责胃的运动动作。胃里有磨胃器，它用内部的三颗牙齿来分解食物，还有幽门，它在食物到达中肠之前过滤食物。STG产生两种有节奏的输出来控制胃和幽门，称为中枢模式发生器（cpg）。STG中的每个细胞都可以参与其中一种或两种节律。这些cpg允许研究神经调节，稳态，细胞和网络变异性，网络发展和网络恢复。对约拿蟹（Cancer borealis）口胃神经系统（STNS）的解剖分为两部分；粗细解剖。在大体解剖中，螃蟹的整个胃都被解剖出来。在精细解剖过程中，使用解剖显微镜和显微解剖工具从胃中取出STNS（见图1）。STNS包括STG、食道神经节（OG）、交节（CoG）以及支配胃肌肉的神经。在这里，我们展示了如何对STNS进行完整的解剖，以准备电生理实验，其中STG中的细胞将从细胞内记录，而周围神经将用于细胞外记录。本文展示了寻找所需神经的适当技术，以及我们对神经节进行脱皮以显示躯体和神经节的技术。
The stomatogastric ganglion (STG) is an excellent model for studying cellular and network interactions because it contains a relatively small number of cells (approximately 25 in C. borealis) which are well characterized. The cells in the STG exhibit a broad range of outputs and are responsible for the motor actions of the stomach. The stomach contains the gastric mill which breaks down food with three internal teeth, and the pylorus which filters the food before it reaches the midgut. The STG produces two rhythmic outputs to control the gastric mill and pylorus known as central pattern generators (CPGs). Each cell in the STG can participate in one or both of these rhythms. These CPGs allow for the study of neuromodulation, homeostasis, cellular and network variability, network development, and network recovery. The dissection of the stomatogastric nervous system (STNS) from the Jonah crab (Cancer borealis) is done in two parts; the gross and fine dissection. In the gross dissection the entire stomach is dissected from the crab. During the fine dissection the STNS is extracted from the stomach using a dissection microscope and micro-dissection tools (see figure 1). The STNS includes the STG, the oesophageal ganglion (OG), and the commissural ganglia (CoG) as well as the nerves that innervate the stomach muscles. Here, we show how to perform a complete dissection of the STNS in preparation for an electrophysiology experiment where the cells in the STG would be recorded from intracellularly and the peripheral nerves would be used for extracellular recordings. The proper technique for finding the desired nerves is shown as well as our technique of desheathing the ganglion to reveal the somata and neuropil.