Effect of magnesium on reactive oxygen species production in the thigh muscles of broiler chickens
Effect of magnesium on reactive oxygen species production in the thigh muscles of broiler chickens
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DOI：
10.1080/00071660601148187
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发表时间：
2007-02-01
影响因子：
2
通讯作者：
Wang, Z.
中科院分区：
文献类型：
--
作者：
Liu, Y. X.;Guo, Y. M.;Wang, Z.
1.本研究旨在探讨镁对肉鸡大腿肌肉中活性氧(ROS)生成的影响。将96只1日龄AA肉鸡随机分为两组，分别饲喂含干物质约1.2g/kg或2.4g mg/kg的低镁日粮和对照日粮。低镁日粮显著提高肉仔鸡大腿肌肉中丙二醛(MDA)含量，降低谷胱甘肽(GSH)含量。低镁组大鼠大腿肌肉匀浆中ROS的生成量显著高于对照组。与对照组相比，低镁组肉鸡肌肉镁含量降低了9.5%。低镁组肉鸡线粒体电子传递链复合体II和III活性分别提高23%和35%。ROS的产生与线粒体电子传递链(ETC)复合体的活性呈显著负相关。低镁日粮不影响肉鸡大腿肌肉中铁(Fe)和钙(Ca)的含量，也不影响大腿肌肉中的不饱和脂肪酸组成(C18：2除外)。低镁日粮可降低肉鸡大腿肌肉中的镁浓度，进而诱导线粒体等酶活性升高，从而增加ROS的产生。这些结果表明，镁至少部分地通过影响ROS的产生来调节大腿肌肉的氧化-抗氧化系统。
1. The objective of the present study was to investigate the effect of magnesium (Mg) on reactive oxygen species (ROS) production in the thigh muscles of broiler chickens. A total of 96 1-d-old male Arbor Acre broiler chickens were randomly allocated into two groups, fed either on low-Mg or control diets containing about 1.2 g/ kg or 2.4 g Mg/kg dry matter.2. The low-Mg diet significantly increased malondialdehyde (MDA) concentration and decreased glutathione (GSH) in the thigh muscles of broiler chickens. ROS production in the thigh muscle homogenate was significantly higher in the low-Mg group than in the control group. Compared with the control, muscle Mg concentration of broiler chickens from the low-Mg group decreased by 9.5%.3. Complex II and III activities of the mitochondrial electron transport chain in broilers on low-Mg diet increased by 23 and 35%, respectively. Significant negative correlations between ROS production and the activities of mitochondrial electron transport chain (ETC) complexes were observed.4. The low-Mg diet did not influence contents of iron (Fe) or calcium (Ca) in the thigh muscles of broiler chickens and did not influence unsaturated fatty acid composition (except C18:2) in the thigh muscles.5. A low-Mg diet decreased Mg concentration in the thigh muscles of broiler chickens and then induced higher activities of mitochondrial ETC, consequently increasing ROS production. These results suggest that Mg modulates the oxidation-anti-oxidation system of the thigh muscles at least partly through affecting ROS production.