IMMUNOCYTOCHEMICAL ANALYSIS OF CELLULAR-COMPONENTS IN ATHEROSCLEROTIC LESIONS - USE OF MONOCLONAL-ANTIBODIES WITH THE WATANABE AND FAT-FED RABBIT
IMMUNOCYTOCHEMICAL ANALYSIS OF CELLULAR-COMPONENTS IN ATHEROSCLEROTIC LESIONS - USE OF MONOCLONAL-ANTIBODIES WITH THE WATANABE AND FAT-FED RABBIT
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DOI：
10.1161/01.atv.6.6.601
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发表时间：
1986-11-01
期刊：
影响因子：
--
通讯作者：
GOWN, AM
中科院分区：
文献类型：
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作者：
TSUKADA, T;ROSENFELD, M;GOWN, AM
我们对Watanabe遗传性高脂血症（WHHL）和脂肪喂养兔动脉粥样硬化病变中平滑肌细胞和巨噬细胞的分布进行了免疫细胞化学研究。我们使用了对肌肉细胞和巨噬细胞特异性的单克隆抗体，后者使用了一种新的巨噬细胞特异性单克隆抗体RAM 11。在大体正常主动脉区域的内皮下观察到散在的巨噬细胞。根据细胞定位的定性方面，将隆起病变分为四个主要组：（1）脂肪条纹，主要由巨噬细胞组成;（2）内膜增厚，主要由平滑肌细胞组成，显示出相当大的形态异质性，并混合有巨噬细胞;（3）早期纤维斑块，特征为平滑肌细胞和巨噬细胞数量大致相等;（4）晚期纤维斑块，由含有扁平平滑肌细胞的纤维帽覆盖在富含巨噬细胞的动脉粥样硬化碎片区组成。这些研究证实了两种家兔模型中病变的性质以及单克隆抗体在分析动脉粥样硬化病变细胞组成中的有用性。
We have performed immunocytochemical investigations into the distribution of smooth muscle cells and macrophages in the atherosclerotic lesions of the Watanabe heritable hyperlipidemic (WHHL) and fat-fed rabbits. We used monoclonal antibodies specific for muscle cells and macrophages, the latter with a new macrophage-specific monoclonal antibody designated RAM11. Scattered macrophages were observed in the subendothelium in areas of grossly normal aorta. Raised lesions were divided into four major groups, based on qualitative aspects of cell localization: (1) fatty streak, composed predominantly of macrophages; (2) intimal thickening, composed predominantly of smooth muscle cells that displayed considerable morphological heterogeneity with an admixture of macrophages; (3) early fibrous plaques, characterized by approximately equal numbers of smooth muscle cells and macrophages; (4) advanced fibrous plaques, composed of a fibrous cap containing flat smooth muscle cells overlaying a macrophage-rich zone of atheromatous debris. These studies demonstrate the nature of the lesions in the two rabbit models and the usefulness of monoclonal antibodies in analyzing the cellular composition of atherosclerotic lesions.