Reproductive and post-embryonic daily rhythm patterns of the malaria vector Anopheles (Kerteszia) cruzii:: Aspects of the life cycle
Reproductive and post-embryonic daily rhythm patterns of the malaria vector Anopheles (Kerteszia) cruzii:: Aspects of the life cycle
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DOI：
10.1080/07420520701282174
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发表时间：
2007-01-01
影响因子：
2.8
通讯作者：
Marques, Mirian David
中科院分区：
文献类型：
--
作者：
Chahad-Ehlers, Samira;Lozovei, Ana Leuch;Marques, Mirian David
雌性按蚊（Kerteszia）cruzii，在巴西南部和东南部的大西洋森林的一些地区的散发性疟疾媒介，被抓获，并在受控条件下进行研究。在实验室中，在25.1 +/- 0.6摄氏度和相对湿度57- 81%的自然光暗周期中进行日常观察。胚后发育，其中包括四个幼虫龄期和蛹，连续观察，其周期，以及时间的组成部分，繁殖，进行了登记。还对女性寿命进行了初步研究。连续三天目视监测产卵、第三和第四龄幼虫蜕皮和化蛹，连续四天监测成虫羽化。通过圆形统计分析结果，并在5%的显著性水平下通过瑞利检验评估无节律的零假设。在捕获的141只雌鱼中，113只（80.14%）存活，79只（69.91%）成功地喂血，提供时间为09：00-10：30 h（早晨）或18：30 - 20：00 h（晚上）。在65次产卵中，36只雌虫共产卵1063粒，其中18只雌虫为碎片产卵。从产卵到成虫羽化的平均时间为30.71 ± 3.57天，幼虫期是胚后发育中最长的阶段。产卵呈日节律，高峰在日落后2 ~ 5 h，即23：24 +/- 3：47 h。血餐的时间并没有改变产卵节律的阶段。最后的幼虫蜕皮，化蛹，和成人出现没有遵循24小时的节奏模式。描述胚胎后发育的时间模式，特别是在病媒的情况下，可以成为确定控制方法的研究中的一个重要工具。
Females of Anopheles (Kerteszia) cruzii, a sporadic malaria vector in some areas of the Atlantic Forest in south and southeastern Brazil, were captured and studied under controlled conditions. In the laboratory, daily observations were conducted in natural light-dark cycles at 25.1 +/- 0.6 degrees C and relative humidity 57-81%. Postembryonic development, which comprises four larval instars and the pupa, was continuously observed, and its cycles, as well as temporal components of reproduction, were registered. A preliminary study on female longevity was also performed. Oviposition, ecdysis from the third and fourth instars larvae, and pupation were visually monitored over three consecutive days and the emergence of adults over four consecutive days. Results were analyzed by circular statistics, and the null hypothesis of the absence of rhythm was assessed by Rayleigh's test at the 5% significance level. From a total of 141 females captured, 113 (80.14%) survived and 79 (69.91%) were successfully fed on blood, offered at one of two time intervals, 09:00-10:30 h ( morning) or 18: 30 20: 00 h ( evening). A total of 36 females laid 1063 eggs in 65 oviposition episodes, and 18 females presented fragmented oviposition. The average duration from egg-laying until adult emergence was 30.71 +/- 3.57 days, the larval stage being the longest in the post-embryonic development. Egg-laying showed a daily rhythm, with a peak at 23:24 +/- 3: 47 h, 2 to 5 h after sunset. The time of the blood meal did not shift the phase of the egg-laying rhythm. The last larval ecdysis, pupation, and adult emergence did not follow a 24 h rhythmic pattern. A description of temporal patterns of post-embryonic development, particularly in the case of vectors, can be an important tool in research to determine methods of control.