KCC2-dependent subcellular ECl difference of ON-OFF retinal ganglion cells in larval zebrafish
KCC2-dependent subcellular ECl difference of ON-OFF retinal ganglion cells in larval zebrafish
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斑马鱼幼虫视网膜神经节细胞ON-OFF依赖的KCC2亚细胞ECl差异
DOI：
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Jiu
中科院分区：
文献类型：
--
作者：
Rong;Shu;Jiu
在许多类型的神经元中发现了Cl−(ECl)逆转电位的亚细胞差异。由于局部ECl在很大程度上决定了附近gaba能/甘氨酸能突触的行为，亚细胞ECl的差异可以有效地调节神经元的计算。视网膜神经节细胞（RGC）分别通过其ON和OFF树突处理ON和OFF视觉信号。因此，研究单个RGCs的ON和OFF树突是否表现出不同的局部ECl是很有趣的。在这里，我们使用斑马鱼幼虫ON-OFF RGCs的体内革兰霉素穿孔补丁记录，分别通过测量光诱发的ON和OFF抑制反应和gaba诱导的体细胞反应来检测ON和OFF树突和体细胞的局部ECl。我们发现在单个RGCs的胞体和树突以及ON和OFF树突之间存在亚细胞ECl差异。这些躯体-树突和树突间的ECl差异依赖于Cl -挤出剂，K+/Cl -共转运体（KCC2），因为它们通过用morpholino寡核苷酸（MOs）下调KCC2的表达或用速尿阻断KCC2的功能而大大减少。因此，我们的研究结果表明，在单个开-关RGCs的开和关树突之间存在kcc2依赖的ECl差异，这可能会不同地影响开和关通路中的视觉处理。
Subcellular difference in the reversal potential of Cl− (ECl) has been found in many types of neurons. As local ECl largely determines the action of nearby GABAergic/glycinergic synapses, subcellular ECl difference can effectively regulate neuronal computation. The ON-OFF retinal ganglion cell (RGC) processes both ON and OFF visual signals via its ON and OFF dendrites, respectively. It is thus interesting to investigate whether the ON and OFF dendrites of single RGCs exhibit different local ECl. Here, using in vivo gramicidin-perforated patch recording in larval zebrafish ON-OFF RGCs, we examine local ECl at the ON and OFF dendrites, and soma through measuring light-evoked ON and OFF inhibitory responses, and GABA-induced response at the soma, respectively. We find there are subcellular ECl differences between the soma and dendrite, as well as between the ON and OFF dendrites of single RGCs. These somato-dendritic and inter-dendritic ECl differences are dependent on the Cl− extruder, K+/Cl− co-transporter (KCC2), because they are largely diminished by down-regulating kcc2 expression with morpholino oligonucleotides (MOs) or by blocking KCC2 function with furosemide. Thus, our findings indicate that there exists KCC2-dependent ECl difference between the ON and OFF dendrites of individual ON-OFF RGCs that may differentially affect visual processing in the ON and OFF pathways.
影响因子：
2.5
作者：
Gao, F;Wu, SM
通讯作者：
Wu, SM