FREEZE-FRACTURE STUDIES ON THE SYNAPSE BETWEEN THE TYPE-I HAIR CELL AND THE CALYCEAL TERMINAL IN THE GUINEA-PIG VESTIBULAR SYSTEM
FREEZE-FRACTURE STUDIES ON THE SYNAPSE BETWEEN THE TYPE-I HAIR CELL AND THE CALYCEAL TERMINAL IN THE GUINEA-PIG VESTIBULAR SYSTEM
复制标题
DOI：
10.1007/bf01208511
复制
发表时间：
1979-01-01
期刊：
影响因子：
--
通讯作者：
BAGGERSJOBACK, D
中科院分区：
文献类型：
--
作者：
GULLEY, RL;BAGGERSJOBACK, D
使用薄切片和冷冻骨折标本，研究了豚鼠前庭上皮中I型毛细胞与囊状神经节细胞外周突的盏终末之间的沉积。化学突触连接是非常罕见的薄切片标本，并没有看到冷冻断裂复制品。毛细胞和毛萼之间无缝隙连接。沿贴壁沿着存在膜紧密贴壁的区域。在这些区域，毛细胞被花萼的胞质突起内陷，两个细胞的质膜仅相隔6-7 nm。不同细胞间紧密贴壁的数量和形态不同。在冷冻断裂复制品，紧密贴壁质膜的毛细胞和花萼没有特殊的膜内颗粒分布在任何膜小叶。在毛细胞的外膜小叶上，一大片宽间距的大颗粒围绕着紧密贴壁的区域。花萼质膜的相应区域在膜小叶上没有特殊的颗粒分布。化学突触连接的稀缺性，细胞之间的间隙连接的情况下，在毛细胞质膜周围区域的紧密膜并置的颗粒的独特的安排可能表明一种不寻常的模式之间的突触传递的I型毛细胞和花萼。
The apposition between type I hair cells and the calyceal terminals of vesticular ganglion cell peripheral processes was studied in the vestibular epithelium of the guinea pig, using thin-sectioned and freeze-fractured specimens. Chemical synaptic junctions were exceedingly rare in thin-sectioned specimens, and were not seen in freeze-fracture replicas. No gap junctions were present between the hair cell and the calyx. There were regions along the apposition where the membranes were closely apposed. At these regions, the hair cell was invaginated by cytoplasmic protrusions of the calyx and the plasmalemmata of the 2 cells were separated by only 6-7 nm. The number and conformation of the close appositions varied between different cells. In freeze-fracture replicas, the closely-apposed plasmalemmata of the hair cell and the calyx had no special distribution of intramembrane particles on either membrane leaflet. On the external membrane leaflet of the hair cell, a large patch of widely-spaced, large particles surrounded the regions of close apposition. The corresponding region of the plasmalemma of the calyx had no special distribution of particles on either membrane leaflet. The scarcity of chemical synaptic junctions, the absence of gap junctions between the cells and the unique arrangement of particles in the hair cell plasmalemma surrounding regions of close membrane apposition may indicate an unusual mode of synaptic transmission between the type I hair cell and the calyx.