Engineering Escherichia coli for fumaric acid production from glycerol
Engineering Escherichia coli for fumaric acid production from glycerol
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改造大肠杆菌用于从甘油生产富马酸。
DOI：
10.1016/j.biortech.2014.09.147
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发表时间：
2014-12-01
影响因子：
11.4
通讯作者：
Zhao, Xueming
中科院分区：
文献类型：
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作者：
Li, Ning;Zhang, Bo;Zhao, Xueming
在本研究中，对先前为从甘油生产琥珀酸而开发的进化突变型大肠杆菌E2进行了工程改造，使其在有氧条件下生产富马酸。通过缺失三种延胡索酸酶，生产出了3.65 g/L的富马酸，产率为0.25 mol/mol甘油，并且积累了大量乙酸作为主要副产物。为了减少乙酸的产生，尝试了几种策略，其中通过过表达磷酸烯醇式丙酮酸羧化酶基因ppc或乙醛酸循环操纵子aceBA来增加回补途径的通量，有效地减少了乙酸并提高了富马酸的产量。在补料分批培养中，所得菌株EF02(pSCppc)从甘油中生产出41.5 g/L的富马酸，达到最大理论产率的70%，总生产率为0.51 g/L/h。（C）2014爱思唯尔有限公司。保留所有权利。
The evolved mutant Escherichia coli E2 previously developed for succinate production from glycerol was engineered in this study for fumaric acid production under aerobic conditions. Through deletion of three fumarases, 3.65 g/L fumaric acid was produced with the yield of 0.25 mol/mol glycerol and a large amount of acetate was accumulated as the main byproduct. In order to reduce acetate production several strategies were attempted, among which increasing the flux of the anaplerotic pathways through overexpression of phosphoenolpyruvate carboxylase gene ppc or the glyoxylate shunt operon aceBA effectively reduced acetate and improved fumaric acid production. In fed-batch culture, the resulting strain EF02(pSCppc) produced 41.5 g/L fumaric acid from glycerol with 70% of the maximum theoretical yield and an overall productivity of 0.51 g/L/h. (C) 2014 Elsevier Ltd. All rights reserved.