Temporal and spatial changes in the expression pattern of multiple red and green subtype opsin genes during zebrafish development
Temporal and spatial changes in the expression pattern of multiple red and green subtype opsin genes during zebrafish development
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DOI：
10.1242/jeb.01532
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发表时间：
2005-04-01
影响因子：
2.8
通讯作者：
Kawamura, S
中科院分区：
文献类型：
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作者：
Takechi, M;Kawamura, S
斑马鱼有两个红色的LWS-1和LWS-2，以及四个绿色的RH2-1，RH2-2，RH2-3和RH2-4，这些视蛋白基因编码具有不同吸收光谱的感光色素。通过基因复制产生视蛋白亚型是鱼类的特征，但目前尚不清楚这些亚型在视网膜中的表达是否在空间上或时间上有所不同。在这里，我们通过原位杂交展示了视蛋白亚型在斑马鱼视网膜中的动态表达模式。红色型Opsins的表达是由波长较短的亚型LWS-2启动的，其次是波长较长的亚型LWS-1。在成人视网膜中，LWS-2在中央至背侧区域表达，LWS-1在腹侧和周围区域表达。绿色型视蛋白的表达模式与红色型视蛋白相似。在成人视网膜中，较短波长亚型(RH2-1和RH2-2)在中央至背侧区域表达，较长波长亚型(RH2-3和RH2-4)在腹侧和周围区域表达。这些结果为后续视蛋白基因调控的研究和利用斑马鱼遗传学的力量探索发育变化的功能基础提供了框架。
Zebrafish have two red, LWS-1 and LWS-2, and four green, RH2-1, RH2-2, RH2-3 and RH2-4, opsin genes encoding photopigments with distinct absorption spectra. Occurrence of opsin subtypes by gene duplication is characteristic of fish but little is known whether the subtypes are expressed differently in the retina, either spatially or temporally. Here we show by in situ hybridization the dynamic expression patterns of the opsin subtypes in the zebrafish retina. Expression of red type opsins is initiated with the shorter-wavelength subtype LWS-2, followed by the longer-wavelength subtype LWS-1. In the adult retina, LWS-2 was expressed in the central to dorsal area and LWS-1 in the ventral and peripheral areas. Expression patterns of green type opsins were similar to those of the red type opsins. The expression started with the shortest wavelength subtype RH2-1 followed by the longer wavelength ones, and in the adult retina, the shorter wavelength subtypes (RH2-1 and RH2-2) were expressed in the central to dorsal area and longer wavelength subtypes (RH2-3 and RH2-4) in the ventral and peripheral areas. These results provide the framework for subsequent studies of opsin gene regulation and for probing functional rationale of the developmental changes by using the power of zebrafish genetics.