DgcA, a diguanylate cyclase from Xanthomonas oryzae pv. oryzae regulates bacterial pathogenicity on rice.
DgcA, a diguanylate cyclase from Xanthomonas oryzae pv. oryzae regulates bacterial pathogenicity on rice.
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DgcA，一种来自米黄单胞菌 pv. 的二鸟苷酸环化酶。
DOI：
10.1038/srep25978
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发表时间：
2016-05-19
影响因子：
4.6
通讯作者：
He J
中科院分区：
文献类型：
--
作者：
Su J;Zou X;Huang L;Bai T;Liu S;Yuan M;Chou SH;He YW;Wang H;He J
水稻白白叶枯病菌（Xoo）是水稻白叶枯病的病原菌，也是世界范围内的重要植物病原菌。它也是研究细菌-植物相互作用的模式生物之一。细菌信号转导途径的最新进展已经确定环二GMP作为控制黄单胞菌致病性的主要第二信使分子。然而，在很大程度上仍然不清楚c-di-GMP如何调节Xoo中细菌毒力因子的分泌。本研究通过对dgcA基因敲除突变株ΔdgcA、dgcA基因过表达株OdgcA、dgcA基因互补株CdgcA和野生型菌株的表型分析，探讨了DgcA（XOO 3988）在Xoo中的重要作用。结果表明，dgcA对细菌的毒力、EPS产生、细菌的自聚集和运动性产生负面影响，但通过调节细胞内c-di-GMP水平而积极触发生物膜的形成。RNA-seq数据进一步确定了349个由DgcA控制的差异表达基因，为更深入地了解Xoo中的信号转导途径提供了基础。总的来说，本研究强调DgcA作为Xoo毒力的主要调节因子，可以作为预防水稻枯萎病的潜在靶标。
Xanthomonas oryzae pv. oryzae (Xoo) is the causal agent of rice blight disease as well as a serious phytopathogen worldwide. It is also one of the model organisms for studying bacteria-plant interactions. Current progress in bacterial signal transduction pathways has identified cyclic di-GMP as a major second messenger molecule in controlling Xanthomonas pathogenicity. However, it still remains largely unclear how c-di-GMP regulates the secretion of bacterial virulence factors in Xoo. In this study, we focused on the important roles played by DgcA (XOO3988), one of our previously identified diguanylate cyclases in Xoo, through further investigating the phenotypes of several dgcA-related mutants, namely, the dgcA-knockout mutant ΔdgcA, the dgcA overexpression strain OdgcA, the dgcA complemented strain CdgcA and the wild-type strain. The results showed that dgcA negatively affected virulence, EPS production, bacterial autoaggregation and motility, but positively triggered biofilm formation via modulating the intracellular c-di-GMP levels. RNA-seq data further identified 349 differentially expressed genes controlled by DgcA, providing a foundation for a more solid understanding of the signal transduction pathways in Xoo. Collectively, the present study highlights DgcA as a major regulator of Xoo virulence, and can serve as a potential target for preventing rice blight diseases.