Spatial Organization of the Cell Cytoplasm by Position-Dependent Phase Separation
Spatial Organization of the Cell Cytoplasm by Position-Dependent Phase Separation
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DOI：
10.1103/physrevlett.111.088101
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发表时间：
2013-08-20
影响因子：
8.6
通讯作者：
Juelicher, Frank
中科院分区：
文献类型：
--
作者：
Lee, Chiu Fan;Brangwynne, Clifford P.;Juelicher, Frank
在不对称细胞分裂过程中，细胞质成分被分离到细胞的两侧。我们讨论了观察到的分离是如何通过由蛋白质浓度梯度控制的位置依赖相分离机制来实现的。我们表明，即使是弱梯度的影响也可以通过相变放大，以实现强偏析。我们将我们的理论与秀丽隐杆线虫胚胎分裂过程中观察到的胚芽颗粒分离进行比较。我们的研究表明，液-液相分离在细胞质的组织中起着关键作用。
During asymmetric cell division, cytoplasmic components are segregated to opposite sides of the cell. We discuss how the observed segregation can be achieved by a position-dependent phase separation mechanism controlled by a protein concentration gradient. We show that effects of even a weak gradient can be amplified by the phase transition to achieve strong segregation. We compare our theory to the segregation of germ granules observed during the divisions in the C. elegans embryo. Our study demonstrates how liquid-liquid phase separation can play a key role in the organization of the cytoplasm.