The coffee genome provides insight into the convergent evolution of caffeine biosynthesis
The coffee genome provides insight into the convergent evolution of caffeine biosynthesis
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DOI：
10.1126/science.1255274
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发表时间：
2014-09-05
期刊：
影响因子：
56.9
通讯作者：
Lashermes, Philippe
中科院分区：
文献类型：
--
作者：
Denoeud, France;Carretero-Paulet, Lorenzo;Lashermes, Philippe
咖啡因其特有的风味、香气和咖啡因的刺激作用而成为一种有价值的饮料作物。我们生成了 Coffea canephora 物种的高质量基因组草图，该草图显示了紫苑被子植物中保守的染色体基因顺序。尽管没有显示在番茄等茄科物种中发现的全基因组三倍体的迹象，但该基因组包括几个物种特异性基因家族的扩展，其中涉及咖啡因生产的N-甲基转移酶（NMT）、防御相关基因以及涉及次级化合物合成的生物碱和类黄酮酶。咖啡因 NMT 的比较分析表明，这些基因通过连续串联复制而扩展，独立于可可和茶的基因，表明双子叶植物中的咖啡因具有多系起源。
Coffee is a valuable beverage crop due to its characteristic flavor, aroma, and the stimulating effects of caffeine. We generated a high-quality draft genome of the species Coffea canephora, which displays a conserved chromosomal gene order among asterid angiosperms. Although it shows no sign of the whole-genome triplication identified in Solanaceae species such as tomato, the genome includes several species-specific gene family expansions, among them N-methyltransferases (NMTs) involved in caffeine production, defense-related genes, and alkaloid and flavonoid enzymes involved in secondary compound synthesis. Comparative analyses of caffeine NMTs demonstrate that these genes expanded through sequential tandem duplications independently of genes from cacao and tea, suggesting that caffeine in eudicots is of polyphyletic origin.