Toll-like receptor and antimicrobial peptide expression in the bovine endometrium
Toll-like receptor and antimicrobial peptide expression in the bovine endometrium
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DOI：
10.1186/1477-7827-6-53
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发表时间：
2008-11-18
影响因子：
4.4
通讯作者：
Sheldon, I. Martin
中科院分区：
文献类型：
--
作者：
Davies, Darren;Meade, Kieran G.;Sheldon, I. Martin
背景：子宫内膜通常被细菌感染，导致牛和人类的子宫出现严重疾病。子宫内膜上皮是粘膜表面抵抗细菌的第一道防线，Toll 样受体 (TLR) 是检测病原体相关分子模式 (PAMP) 的先天免疫系统的关键组成部分。抗菌肽、急性期蛋白和粘蛋白-1 (MUC-1) 还可针对粘膜表面的微生物提供非特异性防御。本研究检测了牛子宫内膜中先天免疫防御的表达，并检验了子宫内膜上皮细胞表达 TLR 家族的功能性受体和防御细菌的非特异性效应分子的假设。方法：使用 RT-PCR 检查牛子宫内膜组织和纯化的原代上皮细胞和基质细胞群体的 TLR 基因表达， 抗菌肽和MUC-1。当细胞用细菌脂多糖 (LPS) 和脂蛋白等细菌 PAMP 处理时，通过评估前列腺素 E-2 和急性期蛋白的分泌来测试功能反应。结果：子宫内膜表达 TLRs 1 至 10，而纯化的上皮细胞群表达 TLRs 1 至 7 和 9，基质细胞表达 TLRs 1 至 7 和 9。 TLR 1 至 4、6、7、9 和 10。TLR 似乎具有上皮细胞响应细菌 PAMP 分泌前列腺素 E-2 的功能。此外，上皮细胞表达抗菌肽，例如气管和舌抗菌肽（TAP和LAP）和MUC-1，当细胞用LPS处理时，这些抗菌肽的表达上调。然而，上皮细胞不表达大量的急性期蛋白触珠蛋白或血清淀粉样蛋白A。结论：上皮细胞在牛子宫内膜先天免疫防御的协调中具有重要作用，并且可能是预防子宫内膜细菌感染的关键。
Background: The endometrium is commonly infected with bacteria leading to severe disease of the uterus in cattle and humans. The endometrial epithelium is the first line of defence for this mucosal surface against bacteria and Toll-like receptors (TLRs) are a critical component of the innate immune system for detection of pathogen associated molecular patterns (PAMPs). Antimicrobial peptides, acute phase proteins and Mucin-1 (MUC-1) also provide non-specific defences against microbes on mucosal surfaces. The present study examined the expression of innate immune defences in the bovine endometrium and tested the hypothesis that endometrial epithelial cells express functional receptors of the TLR family and the non-specific effector molecules for defence against bacteria.Methods: Bovine endometrial tissue and purified populations of primary epithelial and stromal cells were examined using RT-PCR for gene expression of TLRs, antimicrobial peptides and MUC-1. Functional responses were tested by evaluating the secretion of prostaglandin E-2 and acute phase proteins when cells were treated with bacterial PAMPs such as bacterial lipopolysaccharide (LPS) and lipoproteins.Results: The endometrium expressed TLRs 1 to 10, whilst purified populations of epithelial cells expressed TLRs 1 to 7 and 9, and stromal cells expressed TLRs 1 to 4, 6, 7, 9 and 10. The TLRs appear to be functional as epithelial cells secreted prostaglandin E-2 in response to bacterial PAMPs. In addition, the epithelial cells expressed antimicrobial peptides, such as Tracheal and Lingual Antimicrobial Peptides (TAP and LAP) and MUC-1, which were upregulated when the cells were treated with LPS. However, the epithelial cells did not express appreciable amounts of the acute phase proteins haptoglobin or serum amyloid A.Conclusion: Epithelial cells have an essential role in the orchestration of innate immune defence of the bovine endometrium and are likely to be the key to prevention of endometrial infection with bacteria.