Nitric oxide inhibits blue light-specific stomatal opening via abscisic acid signaling pathways in Vicia guard cells.
Nitric oxide inhibits blue light-specific stomatal opening via abscisic acid signaling pathways in Vicia guard cells.
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DOI：
10.1093/pcp/pcm039
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发表时间：
2007-05
影响因子：
4.9
通讯作者：
Xiao Zhang;A. Takemiya;T. Kinoshita;K. Shimazaki
中科院分区：
文献类型：
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作者：
Xiao Zhang;A. Takemiya;T. Kinoshita;K. Shimazaki
最近的研究表明，一氧化氮（NO）是气孔关闭过程中阿坝信号转导的中间产物。然而，NO的气孔开放的影响知之甚少，即使开放和关闭活动决定气孔开度。在这里，我们表明，NO抑制气孔开放特定的蓝光，从而刺激气孔关闭。NO抑制蓝光诱导的气孔开放，但不抑制红光诱导的气孔开放。NO抑制蓝光诱导的H（+）泵和H（+）-ATP酶磷酸化。NO清除剂2-羧基苯基-4，4，5，5-四甲基咪唑啉-1-氧-3-氧化物（c-PTIO）可恢复上述抑制作用。阿坝和过氧化氢（H（2）O（2））以类似于NO的方式抑制所有这些蓝光特异性反应。c-PTIO部分恢复ABA诱导的所有这些开放反应的抑制，但不能恢复H（2）O（2）对这些反应的抑制。阿坝、H_2O_2和NO对保卫细胞蓝光受体Phototropins的磷酸化有轻微的抑制作用。NO既不抑制梭孢霉素诱导的保卫细胞H（+）泵，也不抑制H（+）-ATPase的H（+）转运。结果表明，NO和H（2）O（2）均通过抑制保卫细胞中的光合素和H（+）-ATPase之间的组分来抑制蓝光诱导的H（+）-ATPase的激活，并通过阿坝促进气孔关闭。
Recent evidence suggests that nitric oxide (NO) acts as an intermediate of ABA signal transduction for stomatal closure. However, NO's effect on stomatal opening is poorly understood even though both opening and closing activities determine stomatal aperture. Here we show that NO inhibits stomatal opening specific to blue light, thereby stimulating stomatal closure. NO inhibited blue light-specific stomatal opening but not red light-induced opening. NO inhibited both blue light-induced H(+) pumping and H(+)-ATPase phosphorylation. The NO scavenger 2-carboxyphenyl-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (c-PTIO) restored all these inhibitory effects. ABA and hydrogen peroxide (H(2)O(2)) inhibited all of these blue light-specific responses in a manner similar to NO. c-PTIO partially restored the ABA-induced inhibition of all of these opening responses but did not restore inhibition of the responses by H(2)O(2). ABA, H(2)O(2) and NO had slight inhibitory effects on the phosphorylation of phototropins, which are blue light receptors in guard cells. NO inhibited neither fusicoccin-induced H(+) pumping in guard cells nor H(+) transport by H(+)-ATPase in the isolated membranes. From these results, we conclude that both NO and H(2)O(2) inhibit blue light-induced activation of H(+)-ATPase by inhibiting the component(s) between phototropins and H(+)-ATPase in guard cells and stimulate stomatal closure by ABA.