Detection and typing of Campylobacter jejuni and Campylobacter coli and analysis of indicator organisms in three waterborne outbreaks in Finland
Detection and typing of Campylobacter jejuni and Campylobacter coli and analysis of indicator organisms in three waterborne outbreaks in Finland
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DOI：
10.1128/aem.69.3.1391-1396.2003
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发表时间：
2003-03-01
影响因子：
4.4
通讯作者：
Rautelin, H
中科院分区：
文献类型：
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作者：
Hänninen, ML;Haajanen, H;Rautelin, H
在北欧国家瑞典、挪威和芬兰，与空肠弯曲菌污染饮用水相关的水源性暴发相当常见，在人口稀少的地区，通常使用地下水而不消毒。在潜在来源中很少检测到弯曲杆菌、大肠杆菌或其他大肠菌群。我们研究了芬兰三次由C.空肠弯曲菌的分析所用的样品体积为4，000至20，000 ml;大肠菌群和大肠杆菌的分析所用的样品体积为1至5，000 ml。大肠杆菌，取决于采样地点。从可能的来源（配水系统和环境水源）获得的多个样品和使用大样品体积（几升）增加了检测病原体C的机会。空肠加水。大体积（1，000至2，000 ml）的过滤也增加了大肠菌群和大肠杆菌的检出率。杆菌为了确认饮用水污染和疾病之间的关联，Penner血清分型和脉冲场凝胶电泳（用SmaI和Kpn I消化）的组合被发现是有用的。这种组合可靠地验证了C的相似性或相异性。从患者样本、饮用水和其他环境来源中分离出空肠，从而证实了可能的暴发宿主。
Waterborne outbreaks associated with contamination of drinking water by Campylobacter jejuni are rather common in the Nordic countries Sweden, Norway, and Finland, where in sparsely populated districts groundwater is commonly used without disinfection. Campylobacters, Escherichia coli, or other coliforms have rarely been detected in potential sources. We studied three waterborne outbreaks in Finland caused by C. jejuni and used sample volumes of 4,000 to 20,000 ml for analysis of campylobacters; and sample volumes of 1 to 5,000 ml for analysis of coliforms and E. coli, depending on the sampling site. Multiple samples obtained from possible sources (water distribution systems and environmental water sources) and the use of large sample volumes (several liters) increased the chance of detecting the pathogen C. jejuni in water. Filtration of a large volume (1,000 to 2,000 ml) also increased the rate of detection of coliforms and E. coli. To confirm the association between drinking water contamination and illness, a combination of Penner serotyping and pulsed-field gel electrophoresis (digestion with SmaI and KpnI) was found to be useful. This combination reliably verified similarity or dissimilarity of C. jejuni isolates from patient samples, from drinking water, and from other environmental sources, thus confirming the likely reservoir of an outbreak.