The complete amino acid sequence of the major component myoglobin of Amazon river dolphin (Inia geoffrensis).
The complete amino acid sequence of the major component myoglobin of Amazon river dolphin (Inia geoffrensis).
复制标题
亚马逊河豚 (Inia geoffrensis) 主要成分肌红蛋白的完整氨基酸序列。
DOI：
10.1021/bi00695a018
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发表时间：
1975
期刊：
影响因子：
2.9
通讯作者：
F. Gurd
中科院分区：
文献类型：
--
作者：
F. Dwulet;R. Bogardt;B. Jones;L. Lehman;F. Gurd
通过对亚马逊河海豚（Inia geoffrensis）肌红蛋白主要成分的特异性切割，获得了该蛋白的大肽段，并利用自动测序仪测定了其氨基酸序列。通过用溴化氰在甲硫氨酸残基处切割蛋白质，获得了三种容易分离的肽，并且通过用胰蛋白酶在精氨酸残基处切割甲基乙酰亚胺化的蛋白质，获得了四种肽。从这些肽中完成了超过85%的序列。序列的剩余部分通过用胰蛋白酶将大溴化氰肽片段化而获得。这种蛋白质与普通鼠海豚（Phocoena phocoena）的蛋白质在7个位置不同，与普通海豚（Delphinus delphis）的蛋白质在11个位置不同，与抹香鲸（Physeter catodon）的蛋白质在15个位置不同。通过与抹香鲸肌红蛋白的三维结构的序列的比较，似乎那些接近血红素组的残基是最保守的，其次是那些在非螺旋区域，最后是那些在螺旋段。在这个序列中观察到的所有取代都很容易地适合抹香鲸肌红蛋白的三维结构。
The complete amino acid sequence of the major component myoglobin from Amazon River dolphin, Inia geoffrensis, was determined by specific cleavage of the protein to obtain large peptides which are readily degraded by the automatic sequencer. Three easily separable peptides were obtained by cleaving the protein with cyanogen bromide at the methionine residues and four peptides were obtained by cleaving the methyl-acetimidated protein with trypsin at the arginine residues. From these peptides over 85% of the sequence was completed. The remainder of the sequence was obtained by fragmentation of the large cyanogen bromide peptide with trypsin. This protein differs from that of the common porpoise, Phocoena phocoena, at seven positions, from that of the common dolphin, Delphinus delphis, at 11 positions, and from that of the sperm whale, Physeter catodon, at 15 positions. By comparison of this sequence with the three-dimensional structure of sperm whale myoglobin it appears that those residues close to the heme group are most conserved followed by those in nonhelical regions and lastly by those in the helical segments. All of the substitutions observed in this sequence fit easily into the three-dimensional structure of the sperm whale myoglobin.