Array comparative genomic hybridization and its applications in cancer
Array comparative genomic hybridization and its applications in cancer
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DOI：
10.1038/ng1569
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发表时间：
2005-06-01
期刊：
影响因子：
30.8
通讯作者：
Albertson, DG
中科院分区：
文献类型：
--
作者：
Pinkel, D;Albertson, DG
DNA 拷贝数的改变是改变基因表达和功能的多种方式之一。一些变异存在于正常个体中，另一些变异发生在某些物种的正常过程中，还有一些变异参与引起各种疾病状态。例如，人类发育中的许多缺陷是由于受精前或受精后不久发生的染色体和染色体片段的获得和丢失造成的，而体细胞中发生的 DNA 剂量变化是癌症的常见诱因。检测这些畸变并在更广泛的知识背景下解释它们有助于识别生物过程和疾病中涉及的关键基因和途径。在过去的几年中，芯片比较基因组杂交已经证明了其在分析 DNA 拷贝数变异方面的价值。在这里，我们讨论阵列比较基因组杂交的最新技术及其在癌症中的应用，强调一般概念而不是具体结果。
Alteration in DNA copy number is one of the many ways in which gene expression and function may be modified. Some variations are found among normal individuals, others occur in the course of normal processes in some species and still others participate in causing various disease states. For example, many defects in human development are due to gains and losses of chromosomes and chromosomal segments that occur before or shortly after fertilization, and DNA dosage-alteration changes occurring in somatic cells are frequent contributors to cancer. Detecting these aberrations and interpreting them in the context of broader knowledge facilitates the identification of crucial genes and pathways involved in biological processes and disease. Over the past several years, array comparative genomic hybridization has proven its value for analyzing DNA copy-number variations. Here, we discuss the state of the art of array comparative genomic hybridization and its applications in cancer, emphasizing general concepts rather than specific results.