Annexin-phospholipid interactions. Functional implications.
Annexin-phospholipid interactions. Functional implications.
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DOI：
10.3390/ijms14022652
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发表时间：
2013-01-28
影响因子：
5.6
通讯作者：
Turnay J
中科院分区：
文献类型：
--
作者：
Lizarbe MA;Barrasa JI;Olmo N;Gavilanes F;Turnay J
膜联蛋白构成了一个进化保守的多基因蛋白超家族，其特征是它们能够以钙依赖的方式与生物膜相互作用。除某些单细胞生物外，所有生物都表达它们。脊椎动物膜联蛋白核心由大约70个氨基酸的4个同源结构域（在膜联蛋白A6中为8个）组成，其整体形状为围绕中心亲水性孔的微弯曲环。钙和磷脂结合位点位于凸侧，而n端连接结构域I和IV位于凹侧。n端区在长度和氨基酸序列上具有很大的可变性，对膜联蛋白的稳定性和特异性功能有很大的影响。这些蛋白质主要与酸性磷脂相互作用，如磷脂酰丝氨酸，但在它们对脂质的亲和力和相互作用所需的钙方面存在差异。在体外，膜联蛋白参与了广泛的细胞内和细胞外生物过程，其中大多数与磷脂双层结合的保守能力直接相关：膜运输，膜-细胞骨架锚定，离子通道活性和调节，以及抗炎和抗凝血活性。然而，膜联蛋白的体内生理功能才刚刚开始确立。
Annexins constitute an evolutionary conserved multigene protein superfamily characterized by their ability to interact with biological membranes in a calcium dependent manner. They are expressed by all living organisms with the exception of certain unicellular organisms. The vertebrate annexin core is composed of four (eight in annexin A6) homologous domains of around 70 amino acids, with the overall shape of a slightly bent ring surrounding a central hydrophilic pore. Calcium- and phospholipid-binding sites are located on the convex side while the N-terminus links domains I and IV on the concave side. The N-terminus region shows great variability in length and amino acid sequence and it greatly influences protein stability and specific functions of annexins. These proteins interact mainly with acidic phospholipids, such as phosphatidylserine, but differences are found regarding their affinity for lipids and calcium requirements for the interaction. Annexins are involved in a wide range of intra- and extracellular biological processes in vitro, most of them directly related with the conserved ability to bind to phospholipid bilayers: membrane trafficking, membrane-cytoskeleton anchorage, ion channel activity and regulation, as well as antiinflammatory and anticoagulant activities. However, the in vivo physiological functions of annexins are just beginning to be established.
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影响因子：
4.8
作者：
Brumatti, Gabriela;Sheridan, Clare;Martin, Seamus J.
通讯作者：
Martin, Seamus J.
影响因子：
3.1
作者：
Bauwens, Matthias;De Saint-Hubert, Marijke;Verbruggen, Alfons
通讯作者：
Verbruggen, Alfons
影响因子：
2.9
作者：
Bitto, E;Cho, W
通讯作者：
Cho, W
DOI：
10.1016/s0005-2760(98)00026-5
发表时间：
1998-04-22
期刊：
BIOCHIMICA ET BIOPHYSICA ACTA-LIPIDS AND LIPID METABOLISM
影响因子：
--
作者：
Buckland, AG;Wilton, DC
通讯作者：
Wilton, DC
影响因子：
--
作者：
Bandorowicz-Pikula, Joanna;Wos, Marcin;Pikula, Slawomir
通讯作者：
Pikula, Slawomir