VPS21 controls entry of endocytosed and biosynthetic proteins into the yeast prevacuolar compartment
VPS21 controls entry of endocytosed and biosynthetic proteins into the yeast prevacuolar compartment
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DOI：
10.1091/mbc.11.2.613
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发表时间：
2000-02-01
影响因子：
3.3
通讯作者：
Stevens, TH
中科院分区：
文献类型：
--
作者：
Gerrard, SR;Bryant, NJ;Stevens, TH
酿酒酵母VPS（vacuolar protein sorting，空泡蛋白分选）基因的突变已被用于定义可溶性空泡水解酶从晚期高尔基体到空泡的运输步骤。D类VPS基因包括VPS 21、PEP 12和VPS 45，它们似乎编码参与高尔基体至内体转运的膜融合复合物的组分。Vps 21 p是小Ras样GTP酶的Rab家族的成员，并且显示出与哺乳动物Rab 5蛋白的强同源性，Rab 5蛋白参与内吞作用和早期内体的同型融合。虽然Rab 5和Vps 21 p在序列水平上看起来是同源的，但这两种Rabs的功能是否相似尚不清楚。我们发现，Vps 21 p是一个内体蛋白，参与传递液泡和内吞蛋白质的液泡。液泡和内吞蛋白质在缺乏Vps 21 p功能的细胞中以不同的运输中间体积累。因此，似乎Vps 21 p参与了进入前液泡/晚期内体室的两个运输步骤。
Mutations in the VPS (vacuolar protein sorting) genes of Saccharomyces cerevisae have been used to define the trafficking steps that soluble vacuolar hydrolases take en route from the late Golgi to the vacuole. The class D VPS genes include VPS21, PEP12, and VPS45, which appear to encode components of a membrane fusion complex involved in Golgi-to-endosome transport. Vps21p is a member of the Rab family of small Ras-like GTPases and shows strong homology to the mammalian Rab5 protein, which is involved in endocytosis and the homotypic fusion of early endosomes. Although Rab5 and Vps21p appear homologous at the sequence level, it has not been clear if the functions of these two Rabs are similar. We find that Vps21p is an endosomal protein that is involved in the delivery of vacuolar and endocytosed proteins to the vacuole. Vacuolar and endocytosed proteins accumulate in distinct transport intermediates in cells that lack Vps21p function. Therefore, it appears that Vps21p is involved in two trafficking steps into the prevacuolar/late endosomal compartment.