CHARACTERIZATION OF HAIR FOLLICLE BULGE IN HUMAN FETAL SKIN - THE HUMAN FETAL BULGE IS A POOL OF UNDIFFERENTIATED KERATINOCYTES
CHARACTERIZATION OF HAIR FOLLICLE BULGE IN HUMAN FETAL SKIN - THE HUMAN FETAL BULGE IS A POOL OF UNDIFFERENTIATED KERATINOCYTES
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DOI：
10.1111/1523-1747.ep12326649
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发表时间：
1995-12-01
影响因子：
6.5
通讯作者：
HOLBROOK, KA
中科院分区：
文献类型：
--
作者：
AKIYAMA, M;DALE, BA;HOLBROOK, KA
有人认为毛囊的突起包含一个毛囊干细胞池，它可能是移植物抗宿主病的靶点，也是具有致癌潜力的细胞的来源。虽然在成人卵泡中有微小的肿胀，但在发育中的卵泡中凸起很明显。本文对人胎儿皮肤标本中的膨大进行了研究，超微结构上，膨大细胞，尤其是内部细胞，含有丰富的游离核糖体和糖原颗粒，但几乎没有分化的细胞质。几种特异性抗角蛋白抗体的免疫染色显示，隆起细胞表达复层上皮和单层上皮的角蛋白。免疫组织化学和超微结构标准所确定的黑素细胞和Merkel细胞位于隆起细胞之间。激光共聚焦显微镜显示原始的平滑肌细胞最初直接附着在隆起的球状毛发中段，这是凸起最突出的阶段。K-层粘连蛋白和III型胶原在隆起的真皮-表皮交界处以及球的基质区域和真皮乳头之间有很强的表达。因此，湖南毛囊的隆起不仅是直肌的附着部位，而且是相对未分化的角质形成细胞池。
It has been suggested that the bulge of the hair follicle contains a pool of follicular stem cells that may serve as a target site of graft-versus-host disease and as a source of cells with carcinogenic potential. The bulge is prominent in the developing follicle although it is a subtle swelling in the adult follicle. In this paper, we studied the bulge in human fetal skin specimens, Ultrastructurally, the bulge cells, especially the interior cells, have abundant free ribosomes and glycogen particles, but almost no cytoplasmic organelles indicative of differentiation. Immunostaining with several specific anti-keratin antibodies demonstrated that the bulge cells express keratins of both stratified and simple epithelia. Melanocytes and Merkel cells, defined by immunohistochemical and ultrastructural criteria, are seen among bulge cells, Laser confocal microscopy revealed that primitive smooth muscle cells attached directly to the bulge initially at the mid-bulbous hair peg, the stage when the bulge is most prominent. K-laminin and type VII collagen are strongly expressed in the dermoepidermal junction of the bulge and between the matrix area of the bulb and the dermal papilla. Thus, the bulge of hunan hair follicle is not only an attachment site for arrector pill muscle, but also a pool of keratinocytes that are relatively undifferentiated.