The Zebrafish (Danio rerio) Embryo Model as a Tool to Assess Drinking Water Treatment Efficacy for Freshwater Impacted by Crude Oil Spill.
The Zebrafish (Danio rerio) Embryo Model as a Tool to Assess Drinking Water Treatment Efficacy for Freshwater Impacted by Crude Oil Spill.
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DOI：
10.1002/etc.5472
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发表时间：
2022-11
影响因子：
4.1
通讯作者：
Timme-Laragy AR
中科院分区：
文献类型：
--
作者：
Roy MA;Mohan A;Karasik Y;Tobiason JE;Reckhow DA;Timme-Laragy AR
使用分析化学评估饮用水供应中的溢油缓解效果的传统方法可能会忽略残留的碳氢化合物和未知毒性的处理副产品。斑马鱼（Danio rerio）被用来解决这一限制，通过评估的毒性减少鱼类暴露于实验室溶液中的溶解原油成分处理3毫克/升臭氧（O3），或不使用过氧化物酮为基础的高级氧化过程中使用0.5 M H2 O2/M O3或1 M H2 O2/M O3。原油水混合物（OWM）使用三种混合方案-轨道（OWM-Orb）、快速（OWM-Rap）和叶轮（OWM-Imp）产生，并且含有106-1019 μg/L的溶解的总芳族化合物浓度。在第一个实验中，胚胎在受精后24小时（hpf）暴露于稀释至全强度样品的25%-50%的OWM-Orb或OWM-Rap，并且在第二个实验中，暴露于未处理或处理的50%稀释度的OWM-Imp混合物。毒性特征包括体长、心包面积和鱼鳔膨胀，并且这些根据OWM制备而变化，其中OWM-Rap导致最大毒性，其次是OWM-Imp，然后是OWM-Orb。暴露于50%稀释的OWM-Imp的斑马鱼导致体长缩短6%，心包面积增加83%，并且没有鱼鳔充气，但暴露于单独用O3或用0.5 M H2 O2/M O3处理的50%稀释的OWM-Imp导致正常的斑马鱼发育和54%-57%的平均总芳香破坏。O3 + 1 M H2 O2/M O3发生额外的芳烃去除，但没有进一步衰减对斑马鱼的毒性。这项研究表明，使用斑马鱼作为一个额外的评估组件建模的淡水溢油处理方法的有效性。
Traditional approaches toward evaluating oil spill mitigation effectiveness in drinking water supplies using analytical chemistry can overlook residual hydrocarbons and treatment byproducts of unknown toxicity. Zebrafish (Danio rerio) were used to address this limitation by evaluating the reduction in toxicity to fish exposed to laboratory solutions of dissolved crude oil constituents treated with 3 mg/L ozone (O3) with or without a peroxone-based advanced oxidation process using 0.5 M H2O2/M O3 or 1 M H2O2/M O3. Crude oil water mixtures (OWMs) were generated using three mixing protocols—orbital (OWM-Orb), rapid (OWM-Rap), and impeller (OWM-Imp) and contained dissolved total aromatic concentrations of 106–1019 μg/L. In a first experiment, embryos were exposed at 24 h post fertilization (hpf) to OWM-Orb or OWM-Rap diluted to 25%–50% of full-strength samples and in a second experiment, to untreated or treated OWM-Imp mixtures at 50% dilutions. Toxicity profiles included body length, pericardial area, and swim bladder inflation, and these varied depending on the OWM preparation, with OWM-Rap resulting in the most toxicity, followed by OWM-Imp and then OWM-Orb. Zebrafish exposed to a 50% dilution of OWM-Imp resulted in 6% shorter body length, 83% increased pericardial area, and no swim bladder inflation, but exposure to a 50% dilution of OWM-Imp treated with O3 alone or with 0.5 M H2O2/M O3 resulted in normal zebrafish development and average total aromatic destruction of 54%–57%. Additional aromatic removal occurred with O3 + 1 M H2O2/M O3 but without further attenuation of toxicity to zebrafish. This study demonstrates using zebrafish as an additional evaluation component for modeling the effectiveness of freshwater oil spill treatment methods.
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影响因子：
4.8
作者：
COUMAILLEAU, P;POELLINGER, L;WHITELAW, ML
通讯作者：
WHITELAW, ML
影响因子：
9.8
作者：
El-Din, Mohamed Gamal;Fu, Hongjing;Smith, Daniel W.
通讯作者：
Smith, Daniel W.
影响因子：
4.4
作者：
Goldstone JV;McArthur AG;Kubota A;Zanette J;Parente T;Jönsson ME;Nelson DR;Stegeman JJ
通讯作者：
Stegeman JJ
影响因子：
7.4
作者：
Coutinho, Dayane M.;Franca, Daniela;Azevedo, Debora A.
通讯作者：
Azevedo, Debora A.
影响因子：
9.8
作者：
Fang, Zhi;Huang, Rongfu;El-Din, Mohamed Gamal
通讯作者：
El-Din, Mohamed Gamal