Measurement of the growth of a floc forming bacterium Pseudomonas putida CP1
Measurement of the growth of a floc forming bacterium Pseudomonas putida CP1
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DOI：
10.1007/s10532-006-9054-x
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发表时间：
2007-04-01
期刊：
影响因子：
3.6
通讯作者：
Quilty, B.
中科院分区：
文献类型：
--
作者：
Fakhruddin, A. N. M.;Quilty, B.
恶臭假单胞菌CP1在单氯苯酚上生长时形成菌团，而在苯酚或葡萄糖上不生长。生长在单氯苯酚上的生物体细胞数量增加的同时，干重也减少了。当有机体在营养胁迫下生长时，发现细菌的形状从杆状转变为球状，并伴随着细胞尺寸的减小。这一结果与细胞聚集一起影响了用传统方法(光密度测量、干重测量和平板计数技术)测量系统中的生长参数。利用直接显微计数法监测恶臭假单胞菌CP1的生长，比传统的光密度法、干重测量法和平板计数法更具代表性。
Pseudomonas putida CP1 formed clumps of cells when grown on mono-chlorophenols but not on phenol or glucose. An increase in cell numbers for the organism grown on mono-chlorophenols was accompanied by a decrease in the dry weight. The change in shape of the bacterium from rod shape to coccus shape coupled with a reduction in cell size when the organism was grown under nutritional stress was found. This result together with cell aggregation affected the measurement of growth parameters in the system by conventional methods (optical density measurements, dry weight measurements and the plate count technique). Monitoring growth of Pseudomonas putida CP1 by a direct microscopic count technique was found to be more representative than conventional methods including optical density measurements, dry weight measurements and the plate count technique when grown on phenolics.