Hematopoietic progenitor cell rolling in bone marrow microvessels: parallel contributions by endothelial selectins and vascular cell adhesion molecule 1.
Hematopoietic progenitor cell rolling in bone marrow microvessels: parallel contributions by endothelial selectins and vascular cell adhesion molecule 1.
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DOI：
10.1084/jem.188.3.465
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发表时间：
1998-08-03
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影响因子：
--
通讯作者：
von Andrian UH
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文献类型：
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作者：
Mazo IB;Gutierrez-Ramos JC;Frenette PS;Hynes RO;Wagner DD;von Andrian UH
我们用活体显微镜研究小鼠骨髓（BM）中生理灌注的微血管。骨髓血窦和小静脉，但不是邻近的骨血管，支持造血祖细胞的滚动相互作用。滚动并不涉及L-选择素，但在野生型小鼠与P-或E-选择素的抗体治疗，并在这两个选择素缺乏的小鼠部分减少。选择素非依赖性滚动由α4整合素介导，α 4整合素与内皮血管细胞粘附分子（VCAM）-1相互作用。内皮选择素和VCAM-1的平行作用还不知道是直接将血细胞运输到其他非炎症组织。这种组成型表达的粘附分子的组合，因此可能构成一个BM特异性的祖细胞招募途径类似的血管地址，直接选择性淋巴细胞归巢淋巴器官。
We have used intravital microscopy to study physiologically perfused microvessels in murine bone marrow (BM). BM sinusoids and venules, but not adjacent bone vessels, supported rolling interactions of hematopoietic progenitor cells. Rolling did not involve L-selectin, but was partially reduced in wild-type mice treated with antibodies to P- or E-selectin and in mice that were deficient in these two selectins. Selectin-independent rolling was mediated by α4 integrins, which interacted with endothelial vascular cell adhesion molecule (VCAM)-1. Parallel contribution of the endothelial selectins and VCAM-1 is not known to direct blood cell trafficking to other noninflamed tissues. This combination of constitutively expressed adhesion molecules may thus constitute a BM-specific recruitment pathway for progenitor cells analogous to the vascular addressins that direct selective lymphocyte homing to lymphoid organs.