Purification, physicochemical characterisation and anticancer activity of a polysaccharide from Cyclocarya paliurus leaves
Purification, physicochemical characterisation and anticancer activity of a polysaccharide from Cyclocarya paliurus leaves
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青钱柳叶多糖的纯化、理化表征及抗癌活性
DOI：
10.1016/j.foodchem.2012.09.078
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发表时间：
2013-02-01
期刊：
影响因子：
8.8
通讯作者：
Xie, Ming-Yong
中科院分区：
文献类型：
--
作者：
Xie, Jian-Hua;Liu, Xin;Xie, Ming-Yong
青钱柳[Cylocarya paliurus(Btal.)采用热水提取、乙醇沉淀、脱蛋白、阴离子交换层析等方法分离纯化了苦瓜多糖。采用凝胶渗透色谱(GPC)、气相色谱-质谱(GC-MS)、热重分析(TGA)、傅立叶变换红外光谱(FTIR)、紫外-可见光谱、动态光散射(DLS)和粘度分析等方法对其物化性质进行了表征。采用四甲基偶氮唑蓝(3-(4，5-dimethylthiazol-2-yl)-2，5-diphenyltetrazolium brob，MU)比色法评价其对人胃癌HeLa细胞的抗癌作用。结果表明，CPP的相对分子质量为900 kDa，含有64.8%的总糖、23.5%的糖醛酸、9.26%的蛋白质以及葡萄糖、鼠李糖、阿拉伯糖、木糖、甘露糖和半乳糖6种单糖，摩尔百分比分别为32.7%、9.33%、30.6%、3.48%、10.4%和13.5%。结果表明，CPP对人胃癌HeLa细胞的生长有较强的抑制作用。(C)2012爱思唯尔有限公司。保留所有权利。
A Cyclocarya paliurus (Batal.) Iljinskaja polysaccharide (CPP) was isolated and purified by hot water extraction, ethanol precipitation, deproteinisation and anion-exchange chromatography. Its physicochemical properties were characterised by gel permeation chromatography (GPC), gas chromatography-mass spectrometry (GC-MS), thermal gravimetric analysis (TGA), Fourier transform infrared spectrometry (FTIR), UV-visible spectrophotometry, dynamic light scattering (DLS) and viscometry analysis. The anticancer effect of CPP in human gastric cancer HeLa cells was also evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MU) assay. The results showed that the molecular weight of CPP was 900 kDa, and it contained 64.8% total sugar, 23.5% uronic acid, 9.26% protein, and six kinds of monosaccharides, including glucose, rhamnose, arabinose, xylose, mannose and galactose, with molar percentages of 32.7%, 9.33%, 30.6%, 3.48%, 10.4%, and 13.5%, respectively. Furthermore, the results showed that CPP exhibited a strong inhibition effect on the growth of human gastric cancer HeLa cells. (C) 2012 Elsevier Ltd. All rights reserved.