Identification of phytochemical markers for quality evaluation of tree peony stamen using comprehensive HPLC-based analysis
Identification of phytochemical markers for quality evaluation of tree peony stamen using comprehensive HPLC-based analysis
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基于 HPLC 的综合分析鉴定用于牡丹雄蕊质量评价的植物化学标记
DOI：
10.1016/j.indcrop.2020.112711
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发表时间：
2020
影响因子：
5.9
通讯作者：
Yanlong Zhang
中科院分区：
文献类型：
--
作者：
Lihang Xie;Zhenguo Yan;Mengchen Li;Yao;Aruna Kilaru;L. Niu;Yanlong Zhang
凤丹白和岩海棠雄蕊富含酚类物质，是一种具有多种药理作用的茶叶原料。为探讨牡丹品种雄蕊是否可作为天然抗氧化剂，采用高效液相色谱法（HPLC）测定了35个牡丹品种雄蕊中酚类物质的组成和含量，并结合体外抗氧化特性和化学计量学综合分析，对牡丹品种雄蕊的品质进行了评价。结果表明，牡丹雄蕊酚类物质含量和抗氧化能力具有品种特异性。“紫二桥”雄蕊总酚和黄酮醇含量最高，抗氧化活性最强，而“枫丹白”和P。在测试样本中，rockii处于平均水平以下。此外，37个牡丹品种可分为3大类，其总代谢物含量和抗氧化活性存在显著差异，主要由6种植物化学物质贡献。其中，柚皮苷和苯甲酰芍药苷可作为牡丹雄蕊品质鉴定的重要化学标记物。相关性分析表明，早花品种的雄蕊品质可能较高，其雌蕊隐蔽、重瓣、短穗、心皮多、体积大。同时，筛选出雄蕊富含酚类物质和抗氧化剂特性的品种，以及它们相关的关键表型和植物化学性状。本研究为牡丹花蕊的快速鉴别和高品质鉴定提供了依据，为牡丹花蕊作为功能食品和药用资源的开发利用提供了参考。
Stamen fromPaeonia ostii'Fengdan Bai' andPaeonia rockiiis rich in phenolic compounds and popularly used as tea materials with various pharmaceutical functions. In order to investigate whether stamen from other tree peony cultivars could be used as a natural antioxidant, the quality of stamen from thirty-five cultivars collected from the same garden was evaluated based on their phenolic composition and content by high-performance liquid chromatography analysis andin vitroantioxidant properties coupled with comprehensive chemometrics analysis. The results revealed that phenolic contents and antioxidant capacities of tree peony stamen were unique and cultivar dependent. Stamen from 'Zi Erqiao' exhibited the highest total phenolic and flavonol content, and strongest antioxidant activities, while that of 'Fengdan Bai' andP. rockiiwere at below-average levels among test samples. Further, thirty-seven cultivars of tree peony were divided into three major groups with a significant difference in total metabolites content and antioxidant properties, which were mainly contributed by six phytochemical compounds. Among these, naringin and benzoylpaeoniflorin were found to be critical chemical markers for the identification of tree peony stamen with high quality by chemometric analysis. Moreover, correlation analysis suggested that stamen from the earlier flowering cultivars with the hidden pistil, double petal, shorter thrum, more carpel and volume were possibly of higher quality. Together, cultivars with stamen enriched in phenolics and antioxidants properties, and their relevant critical phenotypic and phytochemical traits were screened out. This study would benefit the rapid identification of tree peony stamen with high quality and provide a valuable reference for its development and utilization as functional foods and pharmaceutical resources.
影响因子：
4.7
作者：
Fujiwara, Hironori;Tabuchi, Masahiro;Arai, Hiroyuki
通讯作者：
Arai, Hiroyuki