Brassinosteroid homeostasis is critical for the functionality of the Medicago truncatula pulvinus
Brassinosteroid homeostasis is critical for the functionality of the Medicago truncatula pulvinus
复制标题
油菜素类固醇稳态对于蒺藜苜蓿的功能至关重要。
DOI：
10.1093/plphys/kiab008
复制
发表时间：
2021-01-25
期刊：
影响因子：
7.4
通讯作者：
Zhou, Chuanen
中科院分区：
文献类型：
--
作者：
Kong, Yiming;Meng, Zhe;Zhou, Chuanen
许多植物种类在白天打开它们的叶子，在晚上像睡觉一样关闭它们。这种叶子的运动被称为夜性，这是一种独特而有趣的现象，几个世纪以来一直引起科学家的极大兴趣。夜动性叶运动广泛存在于豆科植物中，并且是由专门的运动器官枕产生的。虽然一个关键的决定因素枕发育，小叶柄样枕（PLP），已被确定，枕功能的分子遗传基础是很大程度上是未知的。在这里，通过分析敲除突变体桶三叶草（苜蓿），我们表明，无论是改变油菜素内酯（BR）的含量，也不阻止BR信号感知影响枕的决定。然而，BR稳态确实影响夜间叶片运动。叶枕中的BR活性由BR失活基因PHYB ACTIVATION TAGGED SUPPOR1（BAS 1）调节，该基因由PLP直接激活。对M. truncatula和非叶枕形成物种拟南芥和番茄（Solanum lycopersicum）的研究表明，PLP可能作为一个因子，在M.截头体除了暴露BR参与枕的功能，这些结果提供了深入了解物种之间的基因功能是一般的还是专门的。
Many plant species open their leaves during the daytime and close them at night as if sleeping. This leaf movement is known as nyctinasty, a unique and intriguing phenomenon that been of great interest to scientists for centuries. Nyctinastic leaf movement occurs widely in leguminous plants, and is generated by a specialized motor organ, the pulvinus. Although a key determinant of pulvinus development, PETIOLULE-LIKE PULVINUS (PLP), has been identified, the molecular genetic basis for pulvinus function is largely unknown. Here, through an analysis of knockout mutants in barrelclover (Medicago truncatula), we showed that neither altering brassinosteroid (BR) content nor blocking BR signal perception affected pulvinus determination. However, BR homeostasis did influence nyctinastic leaf movement. BR activity in the pulvinus is regulated by a BR-inactivating gene PHYB ACTIVATION TAGGED SUPPRESSOR1 (BAS1), which is directly activated by PLP. A comparative analysis between M. truncatula and the non-pulvinus forming species Arabidopsis and tomato (Solanum lycopersicum) revealed that PLP may act as a factor that associates with unknown regulators in pulvinus determination in M. truncatula. Apart from exposing the involvement of BR in the functionality of the pulvinus, these results have provided insights into whether gene functions among species are general or specialized.